Ser728
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Home > Phosphorylation Site Page: > Ser728  -  MCM3 (human)

Site Information
HtPKtADsQETkEsQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 475062

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 4 , 5 , 6 ) , mutation of modification site ( 2 ) , phospho-antibody ( 7 ) , western blotting ( 2 , 7 )
Disease tissue studied:
breast cancer ( 1 , 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
ATM (mouse) ( 7 ) , ATR (mouse) ( 7 )
Treatments:
KU-55933 ( 7 ) , MLN8054 ( 5 ) , siRNA ( 7 ) , UV ( 7 )

References 

1

Carrier M, et al. (2016) Phosphoproteome and Transcriptome of RA-Responsive and RA-Resistant Breast Cancer Cell Lines. PLoS One 11, e0157290
27362937   Curated Info

2

Han X, et al. (2015) Phosphorylation of Minichromosome Maintenance 3 (MCM3) by Checkpoint Kinase 1 (Chk1) Negatively Regulates DNA Replication and Checkpoint Activation. J Biol Chem 290, 12370-8
25809478   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Yi T, et al. (2014) Quantitative phosphoproteomic analysis reveals system-wide signaling pathways downstream of SDF-1/CXCR4 in breast cancer stem cells. Proc Natl Acad Sci U S A 111, E2182-90
24782546   Curated Info

5

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

6

Matsuoka S, et al. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. Science 316, 1160-6
17525332   Curated Info

7

Shi Y, et al. (2007) Identification of carboxyl-terminal MCM3 phosphorylation sites using polyreactive phosphospecific antibodies. J Biol Chem 282, 9236-43
17244605   Curated Info