Thr172
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Home > Phosphorylation Site Page: > Thr172  -  GAP43 (mouse)

Site Information
VtDAAAttPAAEDAA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449106

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 17 ) , immunoassay ( 1 ) , mass spectrometry ( 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 ) , phospho-antibody ( 1 ) , phosphoamino acid analysis ( 17 ) , phosphopeptide mapping ( 17 ) , western blotting ( 1 )
Disease tissue studied:
neuroblastoma ( 1 , 7 )
Relevant cell line - cell type - tissue:
'brain, cerebral cortex' ( 10 , 14 ) , 'brain, embryonic' ( 1 , 11 , 13 ) , 'brain, forebrain' ( 1 ) , brain ( 5 , 6 , 8 , 9 , 12 , 15 , 16 ) , heart ( 8 ) , HEK293T (epithelial) ( 1 ) , liver ( 4 ) , N1E-115 (neuron) ( 7 ) , Neuro-2a (neuron) ( 1 , 17 ) , neuron-brain ( 1 ) , neuron:postsynaptic density-'brain, hippocampus, CA1 region' ( 3 ) , O1A1 (testicular) ( 17 ) , sciatic nerve ( 1 )

Upstream Regulation
Putative in vivo kinases:
JNK1 (human) ( 1 )
Treatments:
development ( 1 ) , JNK inhibitor V ( 1 ) , JNK inhibitor XVI ( 1 ) , SB203580 ( 1 ) , SP600125 ( 1 ) , U0126 ( 1 )

Downstream Regulation
Effects of modification on biological processes:
cell growth, induced ( 1 ) , cytoskeletal reorganization ( 1 )

References 

1

Okada M, et al. (2021) Phosphorylation of GAP-43 T172 is a molecular marker of growing axons in a wide range of mammals including primates. Mol Brain 14, 66
33832520   Curated Info

2

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

3

Li J, et al. (2016) Long-term potentiation modulates synaptic phosphorylation networks and reshapes the structure of the postsynaptic interactome. Sci Signal 9, rs8
27507650   Curated Info

4

Grimsrud PA, et al. (2012) A quantitative map of the liver mitochondrial phosphoproteome reveals posttranslational control of ketogenesis. Cell Metab 16, 672-83
23140645   Curated Info

5

Trinidad JC, et al. (2012) Global identification and characterization of both O-GlcNAcylation and phosphorylation at the murine synapse. Mol Cell Proteomics 11, 215-29
22645316   Curated Info

6

Goswami T, et al. (2012) Comparative phosphoproteomic analysis of neonatal and adult murine brain. Proteomics 12, 2185-9
22807455   Curated Info

7

Wang Y, et al. (2011) Spatial phosphoprotein profiling reveals a compartmentalized extracellular signal-regulated kinase switch governing neurite growth and retraction. J Biol Chem 286, 18190-201
21454597   Curated Info

8

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

9

Wiƛniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9
20415495   Curated Info

10

Tweedie-Cullen RY, Reck JM, Mansuy IM (2009) Comprehensive mapping of post-translational modifications on synaptic, nuclear, and histone proteins in the adult mouse brain. J Proteome Res 8, 4966-82
19737024   Curated Info

11

Zhou J (2008) CST Curation Set: 4864; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P Antibodies Used to Purify Peptides prior to LCMS: Phospho-Thr-Pro Motif Antibody (polyAB) Cat#: 3003, PTMScan(R) Phospho-Thr-Pro Motif (T*P) Immunoaffinity Beads Cat#: 1996
Curated Info

12

Zhou J (2008) CST Curation Set: 3807; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P , PTMScan(R) PLK Binding Motif (SpTP) Immunoaffinity Beads Cat#: 1995
Curated Info

13

Zhou J (2008) CST Curation Set: 3808; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P , PTMScan(R) PLK Binding Motif (SpTP) Immunoaffinity Beads Cat#: 1995
Curated Info

14

Munton RP, et al. (2007) Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations. Mol Cell Proteomics 6, 283-93
17114649   Curated Info

15

Trinidad JC, et al. (2006) Comprehensive identification of phosphorylation sites in postsynaptic density preparations. Mol Cell Proteomics 5, 914-22
16452087   Curated Info

16

Ballif BA, et al. (2004) Phosphoproteomic analysis of the developing mouse brain. Mol Cell Proteomics 3, 1093-101
15345747   Curated Info

17

Spencer SA, Schuh SM, Liu WS, Willard MB (1992) GAP-43, a protein associated with axon growth, is phosphorylated at three sites in cultured neurons and rat brain. J Biol Chem 267, 9059-64
1533624   Curated Info