Ser897
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Home > Phosphorylation Site Page: > Ser897  -  NMDAR1 (rat)

Site Information
sFKRRRssKDtSTGG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448256

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 19 ) , mutation of modification site ( 1 , 3 , 5 , 19 ) , phospho-antibody ( 2 , 4 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 18 ) , phosphoamino acid analysis ( 19 ) , phosphopeptide mapping ( 4 , 19 ) , western blotting ( 2 , 8 , 10 , 11 , 13 , 16 )
Disease tissue studied:
Parkinson's disease ( 4 )
Relevant cell line - cell type - tissue:
'brain, caudate-putamen' ( 12 ) , 'brain, hippocampus' ( 8 , 15 , 18 ) , 'brain, hippocampus, CA1 region' ( 2 ) , 'brain, nucleus accumbens' ( 12 , 14 ) , 'brain, striatum' ( 4 ) , 'brain, substantia nigra' ( 16 ) , 'neuron, cortical' ( 19 ) , 'neuron, cortical'-brain ( 7 , 13 ) , 'neuron, hippocampal'-brain ( 2 ) , 293 (epithelial) ( 1 , 3 , 5 , 18 , 19 ) , dorsal root ganglia ( 10 ) , QT-6 (fibroblast) ( 18 ) , spinal cord ( 11 )

Upstream Regulation
Putative in vivo kinases:
PKCA (rat) ( 10 )
Kinases, in vitro:
PKACA (cow) ( 18 ) , PKCA (human) ( 17 )
Treatments:
6-OHDA ( 16 ) , 8-Rp-cAMP ( 2 ) , AACOCF3 ( 6 ) , BDNF ( 10 ) , BEL ( 6 ) , capsaicin ( 11 ) , chelerythrine ( 10 , 11 ) , ciclosporin ( 7 ) , colforsin ( 10 , 18 ) , dopaminergic_lesion ( 4 ) , electrical_stimulation ( 10 ) , ethanol ( 13 ) , fearful stimulus ( 2 ) , forced swim test ( 2 ) , glutamic acid ( 10 ) , H-89 ( 10 ) , ischemia/reperfusion ( 15 ) , isoproterenol ( 2 ) , K252a ( 10 ) , levodopa ( 4 ) , morphine ( 12 ) , phorbol_ester ( 10 , 18 ) , propranolol ( 2 ) , Rp-cAMPS ( 13 ) , SCH_23390 ( 2 , 14 ) , SKF38393 ( 14 ) , SKF81297 ( 2 ) , status epilepticus ( 8 ) , U0126 ( 10 )

Downstream Regulation
Effects of modification on NMDAR1:
molecular association, regulation ( 17 ) , receptor desensitization, inhibited ( 1 ) , receptor inactivation, inhibited ( 1 )
Inhibit interaction with:
Calmodulin (rat) ( 17 )

Disease / Diagnostics Relevance
Relevant diseases:
Parkinson's disease ( 4 )

References 

1

Aman TK, et al. (2014) Separate intramolecular targets for protein kinase A control N-methyl-d-aspartate receptor gating and Ca2+ permeability. J Biol Chem 289, 18805-17
24847051   Curated Info

2

Murphy JA, et al. (2014) Phosphorylation of Ser1166 on GluN2B by PKA Is Critical to Synaptic NMDA Receptor Function and Ca2+ Signaling in Spines. J Neurosci 34, 869-79
24431445   Curated Info

3

Xu M, Smothers CT, Woodward JJ (2011) Effects of ethanol on phosphorylation site mutants of recombinant N-methyl-D-aspartate receptors. Alcohol 45, 373-80
21163614   Curated Info

4

Kong M, Ba M, Song L, Liu Z (2009) Comparative effects of acute or chronic administration of levodopa to 6-OHDA-lesioned rats on the expression and phosphorylation of N-methyl-D-aspartate receptor NR1 subunits in the striatum. Neurochem Res 34, 1513-21
19283475   Curated Info

5

Jackson MF, et al. (2006) Protein kinase C enhances glycine-insensitive desensitization of NMDA receptors independently of previously identified protein kinase C sites. J Neurochem 96, 1509-18
16417568   Curated Info

6

Ménard C, Patenaude C, Massicotte G (2005) Phosphorylation of AMPA receptor subunits is differentially regulated by phospholipase A2 inhibitors. Neurosci Lett 389, 51-6
16099093   Curated Info

7

Choe ES, Shin EH, Wang JQ (2005) Inhibition of protein phosphatase 2B upregulates serine phosphorylation of N-methyl-D-aspartate receptor NR1 subunits in striatal neurons in vivo. Neurosci Lett 384, 38-43
15890444   Curated Info

8

Niimura M, et al. (2005) Changes in phosphorylation of the NMDA receptor in the rat hippocampus induced by status epilepticus. J Neurochem 92, 1377-85
15748156   Curated Info

9

Ménard C, et al. (2005) AMPA receptor phosphorylation is selectively regulated by constitutive phospholipase A(2) and 5-lipoxygenase activities. Hippocampus 15, 370-80
15630695   Curated Info

10

Slack SE, et al. (2004) Brain-derived neurotrophic factor induces NMDA receptor subunit one phosphorylation via ERK and PKC in the rat spinal cord. Eur J Neurosci 20, 1769-78
15379998   Curated Info

11

Zou X, Lin Q, Willis WD (2004) Effect of protein kinase C blockade on phosphorylation of NR1 in dorsal horn and spinothalamic tract cells caused by intradermal capsaicin injection in rats. Brain Res 1020, 95-105
15312791   Curated Info

12

Scheggi S, et al. (2004) Dopamine and cyclic AMP-regulated phosphoprotein-32 phosphorylation pattern in cocaine and morphine-sensitized rats. J Neurochem 90, 792-9
15287884   Curated Info

13

Ferrani-Kile K, Randall PK, Leslie SW (2003) Acute ethanol affects phosphorylation state of the NMDA receptor complex: implication of tyrosine phosphatases and protein kinase A. Brain Res Mol Brain Res 115, 78-86
12824058   Curated Info

14

Maldve RE, et al. (2002) DARPP-32 and regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens. Nat Neurosci 5, 641-8
12068305   Curated Info

15

Cheung HH, Teves L, Wallace MC, Gurd JW (2001) Increased phosphorylation of the NR1 subunit of the NMDA receptor following cerebral ischemia. J Neurochem 78, 1179-82
11553692   Curated Info

16

Dunah AW, et al. (2000) Alterations in subunit expression, composition, and phosphorylation of striatal N-methyl-D-aspartate glutamate receptors in a rat 6-hydroxydopamine model of Parkinson's disease. Mol Pharmacol 57, 342-52
10648644   Curated Info

17

Hisatsune C, et al. (1997) Phosphorylation-dependent regulation of N-methyl-D-aspartate receptors by calmodulin. J Biol Chem 272, 20805-10
9252405   Curated Info

18

Tingley WG, et al. (1997) Characterization of protein kinase A and protein kinase C phosphorylation of the N-methyl-D-aspartate receptor NR1 subunit using phosphorylation site-specific antibodies. J Biol Chem 272, 5157-66
9030583   Curated Info

19

Tingley WG, Roche KW, Thompson AK, Huganir RL (1993) Regulation of NMDA receptor phosphorylation by alternative splicing of the C-terminal domain. Nature 364, 70-3
8316301   Curated Info