Ser896
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Home > Phosphorylation Site Page: > Ser896  -  NMDAR1 (rat)

Site Information
ssFKRRRssKDtSTG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448255

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 18 ) , immunoassay ( 3 ) , mutation of modification site ( 4 , 7 , 18 ) , phospho-antibody ( 1 , 2 , 3 , 5 , 6 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 17 ) , phosphoamino acid analysis ( 18 ) , phosphopeptide mapping ( 6 , 18 ) , western blotting ( 1 , 2 , 3 , 5 , 10 , 11 , 13 , 15 )
Disease tissue studied:
colonic inflamation ( 2 ) , Parkinson's disease ( 6 )
Relevant cell line - cell type - tissue:
'brain, hippocampus' ( 3 , 5 , 11 , 14 , 17 ) , 'brain, striatum' ( 6 ) , 'brain, substantia nigra' ( 15 ) , 'neuron, cortical' ( 18 ) , 'neuron, cortical'-brain ( 9 ) , 'neuron, hippocampal, CA1 pyramidal'-brain ( 1 ) , 293 (epithelial) ( 4 , 7 , 17 , 18 ) , neuron-'brain, cerebral cortex' ( 10 ) , QT-6 (fibroblast) ( 17 ) , spinal cord ( 2 , 13 )

Upstream Regulation
Putative in vivo kinases:
PKCA (rat) ( 10 )
Kinases, in vitro:
PKCA (human) ( 16 ) , PKCA (rat) ( 17 )
Treatments:
6-OHDA ( 15 ) , AACOCF3 ( 8 ) , BDNF ( 2 ) , BEL ( 8 ) , bisindolylmaleimide ( 2 ) , capsaicin ( 13 ) , chelerythrine ( 13 ) , ciclosporin ( 9 ) , DHPG ( 10 ) , diabetes ( 3 ) , dopaminergic_lesion ( 6 ) , Go_6976 ( 10 ) , ischemia/reperfusion ( 14 ) , K252a ( 2 ) , levodopa ( 6 , 15 ) , LY294002 ( 2 ) , novel object recognition test ( 1 ) , PAF ( 5 ) , PCA4248 ( 5 ) , PD98059 ( 2 ) , PKC-theta_pseudosubstrate ( 10 ) , PKCalpha_peptide_inhibitor ( 10 ) , PKCbeta1_peptide_inhibitor ( 10 ) , PKCbeta2_peptide_inhibitor ( 10 ) , PKCgamma_peptide_inhibitor ( 10 ) , rottlerin ( 10 ) , status epilepticus ( 11 ) , stress ( 1 ) , tri-nitrobenzene_sulfonic_acid ( 2 ) , U73122 ( 2 ) , wortmannin ( 2 )

Downstream Regulation
Effects of modification on NMDAR1:
molecular association, regulation ( 16 )
Inhibit interaction with:
Calmodulin (rat) ( 16 )

Disease / Diagnostics Relevance
Relevant diseases:
Parkinson's disease ( 6 )

References 

1

Calabrese F, et al. (2017) Chronic Mild Stress-Induced Alterations of Local Protein Synthesis: A Role for Cognitive Impairment. ACS Chem Neurosci 8, 817-825
28094500   Curated Info

2

Liu M, Kay JC, Shen S, Qiao LY (2015) Endogenous BDNF augments NMDA receptor phosphorylation in the spinal cord via PLCγ, PKC, and PI3K/Akt pathways during colitis. J Neuroinflammation 12, 151
26289587   Curated Info

3

Liao MH, et al. (2013) The Disturbance of Hippocampal CaMKII/PKA/PKC Phosphorylation in Early Experimental Diabetes Mellitus. CNS Neurosci Ther 19, 329-36
23490331   Curated Info

4

Xu M, Smothers CT, Woodward JJ (2011) Effects of ethanol on phosphorylation site mutants of recombinant N-methyl-D-aspartate receptors. Alcohol 45, 373-80
21163614   Curated Info

5

Moriguchi S, Shioda N, Yamamoto Y, Fukunaga K (2010) Platelet-activating factor-induced synaptic facilitation is associated with increased calcium/calmodulin-dependent protein kinase II, protein kinase C and extracellular signal-regulated kinase activities in the rat hippocampal CA1 region. Neuroscience 166, 1158-66
20074623   Curated Info

6

Kong M, Ba M, Song L, Liu Z (2009) Comparative effects of acute or chronic administration of levodopa to 6-OHDA-lesioned rats on the expression and phosphorylation of N-methyl-D-aspartate receptor NR1 subunits in the striatum. Neurochem Res 34, 1513-21
19283475   Curated Info

7

Jackson MF, et al. (2006) Protein kinase C enhances glycine-insensitive desensitization of NMDA receptors independently of previously identified protein kinase C sites. J Neurochem 96, 1509-18
16417568   Curated Info

8

Ménard C, Patenaude C, Massicotte G (2005) Phosphorylation of AMPA receptor subunits is differentially regulated by phospholipase A2 inhibitors. Neurosci Lett 389, 51-6
16099093   Curated Info

9

Choe ES, Shin EH, Wang JQ (2005) Inhibition of protein phosphatase 2B upregulates serine phosphorylation of N-methyl-D-aspartate receptor NR1 subunits in striatal neurons in vivo. Neurosci Lett 384, 38-43
15890444   Curated Info

10

Sánchez-Pérez AM, Felipo V (2005) Serines 890 and 896 of the NMDA receptor subunit NR1 are differentially phosphorylated by protein kinase C isoforms. Neurochem Int 47, 84-91
15936117   Curated Info

11

Niimura M, et al. (2005) Changes in phosphorylation of the NMDA receptor in the rat hippocampus induced by status epilepticus. J Neurochem 92, 1377-85
15748156   Curated Info

12

Ménard C, et al. (2005) AMPA receptor phosphorylation is selectively regulated by constitutive phospholipase A(2) and 5-lipoxygenase activities. Hippocampus 15, 370-80
15630695   Curated Info

13

Zou X, Lin Q, Willis WD (2004) Effect of protein kinase C blockade on phosphorylation of NR1 in dorsal horn and spinothalamic tract cells caused by intradermal capsaicin injection in rats. Brain Res 1020, 95-105
15312791   Curated Info

14

Cheung HH, Teves L, Wallace MC, Gurd JW (2001) Increased phosphorylation of the NR1 subunit of the NMDA receptor following cerebral ischemia. J Neurochem 78, 1179-82
11553692   Curated Info

15

Dunah AW, et al. (2000) Alterations in subunit expression, composition, and phosphorylation of striatal N-methyl-D-aspartate glutamate receptors in a rat 6-hydroxydopamine model of Parkinson's disease. Mol Pharmacol 57, 342-52
10648644   Curated Info

16

Hisatsune C, et al. (1997) Phosphorylation-dependent regulation of N-methyl-D-aspartate receptors by calmodulin. J Biol Chem 272, 20805-10
9252405   Curated Info

17

Tingley WG, et al. (1997) Characterization of protein kinase A and protein kinase C phosphorylation of the N-methyl-D-aspartate receptor NR1 subunit using phosphorylation site-specific antibodies. J Biol Chem 272, 5157-66
9030583   Curated Info

18

Tingley WG, Roche KW, Thompson AK, Huganir RL (1993) Regulation of NMDA receptor phosphorylation by alternative splicing of the C-terminal domain. Nature 364, 70-3
8316301   Curated Info