Ser403
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Home > Phosphorylation Site Page: > Ser403  -  MDM4 (human)

Site Information
DLAHSSEsQETISSM   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 474540

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 7 ) , mass spectrometry ( 8 ) , mass spectrometry (in vitro) ( 3 ) , mutation of modification site ( 1 , 2 , 3 , 5 , 6 , 7 , 8 , 9 ) , peptide sequencing ( 8 ) , phospho-antibody ( 4 , 8 , 9 ) , western blotting ( 1 , 3 , 4 , 5 , 7 , 8 )
Disease tissue studied:
bone cancer ( 2 , 4 , 5 , 6 , 7 , 8 ) , breast cancer ( 8 ) , colorectal cancer ( 8 ) , colorectal carcinoma ( 8 ) , lung cancer ( 2 , 3 , 5 , 7 ) , non-small cell lung cancer ( 2 , 3 , 5 , 7 ) , neuroblastoma ( 7 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
ATM (human) ( 2 )
Kinases, in vitro:
ATM (human) ( 9 )
Putative upstream phosphatases:
PPM1D (human) ( 6 )
Phosphatases, in vitro:
PPM1D (human) ( 6 )
Treatments:
adriamycin ( 2 ) , ATM_inhibitor ( 2 ) , neocarzinostatin ( 6 , 9 ) , wortmannin ( 2 )

Downstream Regulation
Effects of modification on MDM4:
intracellular localization ( 7 ) , molecular association, regulation ( 1 , 2 , 6 , 7 ) , protein conformation ( 1 , 2 ) , protein degradation ( 5 , 6 , 8 , 9 ) , ubiquitination ( 5 , 6 , 7 )
Effects of modification on biological processes:
translation, induced ( 2 )
Induce interaction with:
MDM2 (human) ( 2 , 6 )
Inhibit interaction with:
MDM2 (human) ( 7 ) , USP7 (human) ( 6 ) , p53 (human) ( 7 )

References 

1

Medina-Medina I, et al. (2016) Allosteric Interactions by p53 mRNA Govern HDM2 E3 Ubiquitin Ligase Specificity under Different Conditions. Mol Cell Biol 36, 2195-205
27215386   Curated Info

2

Malbert-Colas L, et al. (2014) HDMX Folds the Nascent p53 mRNA following Activation by the ATM Kinase. Mol Cell 54, 500-11
24813712   Curated Info

3

He G, et al. (2014) AMP-Activated Protein Kinase Induces p53 by Phosphorylating MDMX and Inhibiting Its Activity. Mol Cell Biol 34, 148-57
24190973   Curated Info

4

Lu Z, et al. (2013) Protein phosphatase 1 inhibits p53 signaling by dephosphorylating and stabilizing Mdmx. Cell Signal 25, 796-804
23277204   Curated Info

5

Li X, et al. (2012) Abnormal MDMX degradation in tumor cells due to ARF deficiency. Oncogene 31, 3721-32
22120712   Curated Info

6

Zhang X, et al. (2009) Phosphorylation and degradation of MdmX is inhibited by Wip1 phosphatase in the DNA damage response. Cancer Res 69, 7960-8
19808970   Curated Info

7

Ohtsubo C, et al. (2009) Cytoplasmic tethering is involved in synergistic inhibition of p53 by Mdmx and Mdm2. Cancer Sci 100, 1291-9
19432880   Curated Info

8

Chen L, et al. (2005) ATM and Chk2-dependent phosphorylation of MDMX contribute to p53 activation after DNA damage. EMBO J 24, 3411-22
16163388   Curated Info

9

Pereg Y, et al. (2005) Phosphorylation of Hdmx mediates its Hdm2- and ATM-dependent degradation in response to DNA damage. Proc Natl Acad Sci U S A 102, 5056-61
15788536   Curated Info