Lys120

Site Information
FLhSGTAkSVTCTyS SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 474514

In vivo Characterization
Methods used to characterize site in vivo:	3H acetate labeling ( 32 ) , electrophoretic mobility shift ( 3 ) , flow cytometry ( 3 , 4 , 5 ) , immunoassay ( 13 , 24 ) , immunoprecipitation ( 2 , 3 , 4 , 5 , 6 , 10 , 20 , 21 , 23 , 24 , 25 , 31 , 33 , 38 ) , mass spectrometry ( 2 , 4 , 13 , 20 , 37 , 39 ) , mass spectrometry (in vitro) ( 14 , 28 ) , modification-specific antibody ( 3 , 7 , 10 , 11 , 13 , 14 , 16 , 17 , 18 , 21 , 22 , 23 , 24 , 25 , 27 , 29 , 31 , 32 , 33 , 38 , 39 ) , mutation of modification site ( 3 , 4 , 5 , 12 , 13 , 14 , 15 , 17 , 18 , 19 , 20 , 22 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 39 ) , phospho-antibody ( 19 , 31 ) , western blotting ( 2 , 3 , 4 , 5 , 7 , 10 , 11 , 13 , 14 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 27 , 29 , 31 , 32 , 38 , 39 )
Disease tissue studied:	bladder cancer ( 34 ) , bone cancer ( 3 , 4 , 6 , 12 , 14 , 16 , 20 , 21 , 22 , 25 , 33 , 38 ) , osteosarcoma ( 12 ) , brain cancer ( 34 ) , glioblastoma ( 34 ) , glioma ( 34 ) , breast cancer ( 13 , 24 , 31 , 33 , 39 ) , colorectal cancer ( 6 , 14 , 18 , 19 , 20 , 21 , 27 , 29 , 31 , 37 ) , colorectal carcinoma ( 6 , 14 , 18 , 19 , 20 , 21 , 27 , 29 , 31 , 37 ) , liver cancer ( 14 , 22 , 34 ) , lung cancer ( 3 , 5 , 6 , 13 , 14 , 15 , 18 , 20 , 21 , 25 , 30 , 31 , 32 , 33 , 36 , 37 , 39 ) , non-small cell lung cancer ( 3 , 5 , 6 , 13 , 14 , 15 , 18 , 20 , 21 , 25 , 30 , 31 , 32 , 33 , 36 , 37 , 39 ) , non-small cell lung adenocarcinoma ( 13 ) , ovarian cancer ( 10 ) , prostate cancer ( 29 , 33 , 39 ) , melanoma skin cancer ( 3 )
Relevant cell line - cell type - tissue:	'stem, embryonic' ( 17 ) , 293 (epithelial) ( 2 ) , A375 (melanocyte) ( 3 ) , A549 (pulmonary) ( 13 ) , BJ (fibroblast) ( 23 ) , BOSC ( 24 ) , DU 145 (prostate cell) ( 29 ) , E.coli (bacterial) ( 28 ) , EJ (bladder cell) ( 11 ) , HCT116 (intestinal) ( 6 , 14 , 18 , 19 , 20 , 21 , 27 , 29 , 31 , 37 ) , HEK293T (epithelial) ( 4 , 13 , 31 , 32 ) , HepG2 (hepatic) ( 22 , 34 ) , IEC-6 (epithelial) ( 7 ) , LNCaP (prostate cell) ( 29 , 33 , 39 ) , MCF-7 (breast cell) ( 13 , 24 , 31 , 33 , 39 ) , MEF (fibroblast) ( 3 , 24 ) , MEF-3T3 ( 13 ) , MHM (bone cell) ( 12 ) , NCI-H1299 (pulmonary) ( 3 , 5 , 6 , 13 , 14 , 15 , 18 , 20 , 21 , 25 , 30 , 31 , 32 , 33 , 36 , 37 , 39 ) , neuron ( 2 ) , OVISE (ovarian) ( 10 ) , OVTOKO (ovarian) ( 10 ) , PC3 (prostate cell) ( 29 ) , RMG1 (ovarian) ( 10 ) , Saos-2 (bone cell) ( 6 , 22 ) , SMMC7721 (hepatocyte) ( 14 ) , stem-colon ( 7 ) , T24 (bladder cell) ( 34 ) , TOV21G (ovarian) ( 10 ) , U2OS (bone cell) ( 3 , 4 , 12 , 14 , 16 , 20 , 21 , 22 , 25 , 33 , 38 ) , U87MG (glial) ( 34 )

Upstream Regulation
Regulatory protein:	BTG2 (human) ( 11 ) , CCDC8 (human) ( 25 ) , HRas (human) ( 23 ) , ING5 (human) ( 22 ) , MKK3 (human) ( 16 ) , MKK6 (human) ( 16 ) , MYST2 (human) ( 39 ) , MYST3 (human) ( 24 ) , Oct4 (human) ( 17 ) , p16-INK4A (human) ( 38 ) , P38A (human) ( 16 ) , P38B (human) ( 16 ) , PDCD5 (human) ( 22 ) , PML (human) ( 24 ) , RNF8 (human) ( 4 ) , Tip60 (human) ( 4 , 7 , 23 ) , TRIM29 (human) ( 27 ) , UHRF1 (human) ( 21 )
Treatments:	actinomycin_D ( 14 ) , ACY1215 ( 10 ) , adriamycin ( 16 , 22 ) , auranofin ( 7 ) , butyrate ( 13 ) , cisplatin ( 12 ) , CPT ( 18 ) , doxycycline ( 3 ) , etoposide ( 4 , 19 ) , ionizing_radiation ( 7 , 16 , 24 , 29 , 39 ) , KN-92 ( 19 ) , KN-93 ( 19 , 20 ) , linsitinib ( 12 ) , MS275 ( 31 ) , nicotinamide ( 3 , 21 , 25 , 37 ) , nutlin-3 ( 18 , 31 ) , retinoic_acid ( 17 ) , SB203580 ( 16 ) , siRNA ( 13 , 14 , 16 , 18 , 21 , 22 , 24 , 25 , 32 , 39 ) , tiron ( 19 ) , trichostatin_A ( 3 , 21 , 25 , 37 ) , UV ( 27 , 38 , 39 ) , valproic acid ( 29 )

Upstream Regulation

Regulatory protein:

BTG2 (human) ( 11 ) , CCDC8 (human) ( 25 ) , HRas (human) ( 23 ) , ING5 (human) ( 22 ) , MKK3 (human) ( 16 ) , MKK6 (human) ( 16 ) , MYST2 (human) ( 39 ) , MYST3 (human) ( 24 ) , Oct4 (human) ( 17 ) , p16-INK4A (human) ( 38 ) , P38A (human) ( 16 ) , P38B (human) ( 16 ) , PDCD5 (human) ( 22 ) , PML (human) ( 24 ) , RNF8 (human) ( 4 ) , Tip60 (human) ( 4 , 7 , 23 ) , TRIM29 (human) ( 27 ) , UHRF1 (human) ( 21 )

Treatments:

actinomycin_D ( 14 ) , ACY1215 ( 10 ) , adriamycin ( 16 , 22 ) , auranofin ( 7 ) , butyrate ( 13 ) , cisplatin ( 12 ) , CPT ( 18 ) , doxycycline ( 3 ) , etoposide ( 4 , 19 ) , ionizing_radiation ( 7 , 16 , 24 , 29 , 39 ) , KN-92 ( 19 ) , KN-93 ( 19 , 20 ) , linsitinib ( 12 ) , MS275 ( 31 ) , nicotinamide ( 3 , 21 , 25 , 37 ) , nutlin-3 ( 18 , 31 ) , retinoic_acid ( 17 ) , SB203580 ( 16 ) , siRNA ( 13 , 14 , 16 , 18 , 21 , 22 , 24 , 25 , 32 , 39 ) , tiron ( 19 ) , trichostatin_A ( 3 , 21 , 25 , 37 ) , UV ( 27 , 38 , 39 ) , valproic acid ( 29 )

Downstream Regulation
Effects of modification on p53:	acetylation ( 2 , 5 , 6 ) , activity, induced ( 14 , 17 ) , intracellular localization ( 20 , 30 ) , molecular association, regulation ( 6 , 13 , 17 , 18 , 22 , 28 , 39 ) , protein stabilization ( 17 )
Effects of modification on biological processes:	apoptosis, altered ( 6 ) , apoptosis, induced ( 2 , 4 , 7 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 18 , 19 , 20 , 21 , 22 , 25 , 27 , 29 , 31 , 32 , 33 , 37 , 39 ) , carcinogenesis, inhibited ( 11 , 27 ) , cell cycle regulation ( 39 ) , cell differentiation, induced ( 17 ) , cell growth, inhibited ( 1 , 11 , 21 , 27 ) , DNA repair, inhibited ( 4 ) , signaling pathway regulation ( 16 ) , transcription, altered ( 6 , 34 , 36 , 37 ) , transcription, induced ( 1 , 3 , 5 , 12 , 13 , 14 , 16 , 17 , 19 , 20 , 21 , 24 , 32 , 35 , 39 )
Induce interaction with:	DDX5 (human) ( 18 ) , DNA ( 13 , 18 , 22 , 28 , 39 )
Inhibit interaction with:	MDM2 (human) ( 17 )

Disease / Diagnostics Relevance
Relevant diseases:	ovarian cancer ( 10 )

References
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28	Arbely E, et al. (2011) Acetylation of lysine 120 of p53 endows DNA-binding specificity at effective physiological salt concentration. Proc Natl Acad Sci U S A 108, 8251-6 21525412 Curated Info
29	Chen X, Wong JY, Wong P, Radany EH (2011) Low-dose valproic acid enhances radiosensitivity of prostate cancer through acetylated p53-dependent modulation of mitochondrial membrane potential and apoptosis. Mol Cancer Res 9, 448-61 21303901 Curated Info
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32	Li X, et al. (2009) Two mammalian MOF complexes regulate transcription activation by distinct mechanisms. Mol Cell 36, 290-301 19854137 Curated Info
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34	Shinmen N, et al. (2009) Activation of NFAT signal by p53-K120R mutant. FEBS Lett 583, 1916-22 19416725 Curated Info
35	Wu SY, Chiang CM (2009) Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding. EMBO J 28, 1246-59 19339993 Curated Info
36	Tian C, et al. (2009) KRAB-type zinc-finger protein Apak specifically regulates p53-dependent apoptosis. Nat Cell Biol 11, 580-91 19377469 Curated Info
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39	Sykes SM, et al. (2006) Acetylation of the p53 DNA-binding domain regulates apoptosis induction. Mol Cell 24, 841-51 17189187 Curated Info