Ser198

Site Information
TRKsAPssPtLDCEK SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 474508

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ) , mutation of modification site ( 13 ) , phospho-antibody ( 13 ) , western blotting ( 13 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 3 , 6 ) , 'brain, cerebellum' ( 12 ) , 'brain, cerebral cortex' ( 12 ) , 'brain, hippocampus, dentate gyrus' ( 12 ) , 'brain, midbrain' ( 12 ) , 'fat, brown' ( 11 ) , 3T3 (fibroblast) ( 13 ) , brain ( 7 , 8 , 11 ) , COS (fibroblast) ( 13 ) , heart ( 11 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 4 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 4 ) , HL-1 (myocyte) ( 4 ) , kidney ( 11 ) , liver ( 1 , 5 , 11 ) , lung ( 11 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 9 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 10 ) , pancreas ( 11 ) , spleen ( 11 )

In vivo Characterization

Methods used to characterize site in vivo:

mass spectrometry ( 1 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ) , mutation of modification site ( 13 ) , phospho-antibody ( 13 ) , western blotting ( 13 )

Relevant cell line - cell type - tissue:

'3T3-L1, differentiated' (adipocyte) ( 3 , 6 ) , 'brain, cerebellum' ( 12 ) , 'brain, cerebral cortex' ( 12 ) , 'brain, hippocampus, dentate gyrus' ( 12 ) , 'brain, midbrain' ( 12 ) , 'fat, brown' ( 11 ) , 3T3 (fibroblast) ( 13 ) , brain ( 7 , 8 , 11 ) , COS (fibroblast) ( 13 ) , heart ( 11 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 4 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 4 ) , HL-1 (myocyte) ( 4 ) , kidney ( 11 ) , liver ( 1 , 5 , 11 ) , lung ( 11 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 9 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 10 ) , pancreas ( 11 ) , spleen ( 11 )

Upstream Regulation
Kinases, in vitro:	CDK5 (human) ( 14 )

Downstream Regulation
Effects of modification on NDEL1:	activity, induced ( 13 ) , intracellular localization ( 13 )
Effects of modification on biological processes:	cell motility, altered ( 13 )

References
1	Robles MS, Humphrey SJ, Mann M (2017) Phosphorylation Is a Central Mechanism for Circadian Control of Metabolism and Physiology. Cell Metab 25, 118-127 27818261 Curated Info
2	Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250 27841257 Curated Info
3	Minard AY, et al. (2016) mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Rep 17, 29-36 27681418 Curated Info
4	Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45 25162660 Curated Info
5	Wilson-Grady JT, Haas W, Gygi SP (2013) Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation. Methods 61, 277-86 23567750 Curated Info
6	Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20 23684622 Curated Info
7	Trinidad JC, et al. (2012) Global identification and characterization of both O-GlcNAcylation and phosphorylation at the murine synapse. Mol Cell Proteomics 11, 215-29 22645316 Curated Info
8	Goswami T, et al. (2012) Comparative phosphoproteomic analysis of neonatal and adult murine brain. Proteomics 12, 2185-9 22807455 Curated Info
9	Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22 21659604 Curated Info
10	Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6 21659605 Curated Info
11	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
12	Trinidad JC, et al. (2008) Quantitative analysis of synaptic phosphorylation and protein expression. Mol Cell Proteomics 7, 684-96 18056256 Curated Info
13	Shen Y, et al. (2008) Nudel binds Cdc42GAP to modulate Cdc42 activity at the leading edge of migrating cells. Dev Cell 14, 342-53 18331715 Curated Info
14	Mori D, et al. (2007) NDEL1 phosphorylation by Aurora-A kinase is essential for centrosomal maturation, separation, and TACC3 recruitment. Mol Cell Biol 27, 352-67 17060449 Curated Info