Tyr259
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Home > Phosphorylation Site Page: > Tyr259  -  PHB (human)

Site Information
SRSRNItyLPAGQsV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 6374000

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 4 , 5 ) , mass spectrometry ( 3 ) , mass spectrometry (in vitro) ( 1 ) , mutation of modification site ( 1 , 2 , 4 , 5 ) , phospho-antibody ( 1 , 2 , 4 ) , western blotting ( 1 , 2 , 4 , 5 )
Disease tissue studied:
breast cancer ( 4 ) , cervical cancer ( 4 ) , cervical squamous cell carcinoma ( 4 ) , lung cancer ( 2 , 3 , 4 ) , non-small cell lung cancer ( 2 , 4 ) , non-small cell lung adenocarcinoma ( 2 , 3 ) , ovarian cancer ( 1 ) , prostate cancer ( 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
ERas (human) ( 4 )
Putative in vivo kinases:
Kit (human) ( 1 )
Kinases, in vitro:
Kit (human) ( 1 )
Treatments:
imatinib ( 1 ) , insulin ( 5 ) , siRNA ( 1 )

Downstream Regulation
Effects of modification on PHB:
intracellular localization ( 4 ) , molecular association, regulation ( 1 , 4 ) , O-GlcNAc glycosylation ( 5 ) , phosphorylation ( 1 , 4 ) , protein stabilization ( 1 )
Effects of modification on biological processes:
carcinogenesis, induced ( 1 , 4 ) , cell motility, induced ( 1 , 2 , 4 ) , signaling pathway regulation ( 1 , 4 ) , transcription, induced ( 1 )
Induce interaction with:
NOTCH3 (human) ( 1 ) , RAF1 (human) ( 4 )

Disease / Diagnostics Relevance
Relevant diseases:
cervical adenocarcinoma ( 4 ) , ovarian cancer ( 1 )

References 

1

Fang CH, Lin YT, Liang CM, Liang SM (2020) A novel c-Kit/phospho-prohibitin axis enhances ovarian cancer stemness and chemoresistance via Notch3-PBX1 and β-catenin-ABCG2 signaling. J Biomed Sci 27, 42
32169072   Curated Info

2

Ho MY, Liang CM, Liang SM (2015) MIG-7 and phosphorylated prohibitin coordinately regulate lung cancer invasion/metastasis. Oncotarget 6, 381-93
25575814   Curated Info

3

Tsai CF, et al. (2015) Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics. Nat Commun 6, 6622
25814448   Curated Info

4

Chiu CF, et al. (2013) Raf activation by Ras and promotion of cellular metastasis require phosphorylation of prohibitin in the raft domain of the plasma membrane. Oncogene 32, 777-87
22410782   Curated Info

5

Ande SR, Moulik S, Mishra S (2009) Interaction between O-GlcNAc Modification and Tyrosine Phosphorylation of Prohibitin: Implication for a Novel Binary Switch. PLoS One 4, e4586
19238206   Curated Info