Tyr805
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Home > Phosphorylation Site Page: > Tyr805  -  VCP (mouse)

Site Information
EDNDDDLyG______   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449060
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 14 ) , immunoprecipitation ( 13 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 7 , 9 , 10 , 11 , 12 ) , mutation of modification site ( 1 , 8 , 13 ) , phospho-antibody ( 1 , 13 ) , phosphopeptide mapping ( 14 ) , western blotting ( 1 )
Disease tissue studied:
leukemia ( 1 , 10 ) , acute myelogenous leukemia ( 1 ) , acute myeloblastic leukemia, with granulocytic maturation (M2) ( 1 ) , chronic myelogenous leukemia ( 1 , 10 ) , lung cancer ( 1 ) , non-small cell lung cancer ( 1 )
Relevant cell line - cell type - tissue:
'stem, mesenchymal' ( 5 ) , 293 (epithelial) ( 1 , 13 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 13 ) , 3T3 (fibroblast) [Src (human)] ( 9 ) , 3T3 (fibroblast) ( 7 , 11 , 12 ) , A431 (epithelial) ( 13 ) , BaF3 ('B lymphocyte, precursor') ( 10 ) , BW125.1C (T lymphocyte) ( 14 ) , COS (fibroblast) ( 13 ) , HEK293T (epithelial) ( 8 ) , HepG2 (hepatic) ( 13 ) , Jurkat (T lymphocyte) ( 8 ) , K562 (erythroid) ( 1 ) , Kasumi-1 (myeloid) ( 1 ) , liver ( 2 , 3 , 4 ) , MDCK (epithelial) ( 13 ) , MEF (fibroblast) ( 1 ) , NCI-H1299 (pulmonary) ( 1 ) , REF52 (fibroblast) ( 13 ) , Saos-2 (bone cell) ( 13 )

Upstream Regulation
Putative in vivo kinases:
Src (human) ( 1 )
Kinases, in vitro:
Src (human) ( 1 )
Putative upstream phosphatases:
PTP4A2 (human) ( 1 ) , PTPN3 (human) ( 13 )
Phosphatases, in vitro:
PTP1B (human) ( 1 )
Treatments:
antibody ( 14 ) , LLOMe ( 1 ) , siRNA ( 1 ) , vanadate ( 1 )

Downstream Regulation
Effects of modification on VCP:
molecular association, regulation ( 1 , 6 , 8 ) , ubiquitination ( 1 )
Effects of modification on biological processes:
autophagy, inhibited ( 1 )
Induce interaction with:
TSAd (human) ( 8 )
Inhibit interaction with:
NGLY1 (mouse) ( 6 ) , PLAA (human) ( 1 ) , UBXN6 (human) ( 1 )

References 

1

Bai Y, et al. (2023) PTP4A2 promotes lysophagy by dephosphorylation of VCP/p97 at Tyr805. Autophagy 19, 1562-1581
36300783   Curated Info

2

Guo A (2009) CST Curation Set: 7791; Year: 2009; Biosample/Treatment: tissue, liver(STK11)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

3

Guo A (2009) CST Curation Set: 7792; Year: 2009; Biosample/Treatment: tissue, liver(STK11)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

4

Guo A (2009) CST Curation Set: 7802; Year: 2009; Biosample/Treatment: tissue, liver(STK11)/Metformin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

5

Yu J (2007) CST Curation Set: 3379; Year: 2007; Biosample/Treatment: cell line, Mouse Mesenchymal Stem Cells/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Zhao G, et al. (2007) Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Proc Natl Acad Sci U S A 104, 8785-90
17496150   Curated Info

7

Guo A (2005) CST Curation Set: 632; Year: 2005; Biosample/Treatment: cell line, 3T3/EGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

Marti F, King PD (2005) The p95-100 kDa ligand of the T cell-specific adaptor (TSAd) protein Src-homology-2 (SH2) domain implicated in TSAd nuclear import is p97 Valosin-containing protein (VCP). Immunol Lett 97, 235-43
15752563   Curated Info

9

Rush J, et al. (2005) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. Nat Biotechnol 23, 94-101
15592455   Curated Info

10

Goss V (2004) CST Curation Set: 383; Year: 2004; Biosample/Treatment: cell line, BaF3/Serum starved; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

11

Moritz A (2003) CST Curation Set: 151; Year: 2003; Biosample/Treatment: cell line, 3T3/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

12

Moritz A (2003) CST Curation Set: 91; Year: 2003; Biosample/Treatment: cell line, 3T3/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

13

Zhang SH, Liu J, Kobayashi R, Tonks NK (1999) Identification of the cell cycle regulator VCP (p97/CDC48) as a substrate of the band 4.1-related protein-tyrosine phosphatase PTPH1. J Biol Chem 274, 17806-12
10364224   Curated Info

14

Egerton M, Samelson LE (1994) Biochemical characterization of valosin-containing protein, a protein tyrosine kinase substrate in hematopoietic cells. J Biol Chem 269, 11435-41
8157674   Curated Info

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