Thr73
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Home > Phosphorylation Site Page: > Thr73  -  PPP1R2 (human)

Site Information
MKIDEPstPyHSMMG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449051

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 8 ) , mutation of modification site ( 3 , 4 , 6 , 8 ) , phospho-antibody ( 1 , 5 , 6 ) , phosphoamino acid analysis ( 8 ) , western blotting ( 1 , 4 , 5 , 8 )
Disease tissue studied:
cervical cancer ( 5 ) , cervical adenocarcinoma ( 5 ) , colorectal cancer ( 1 ) , colorectal carcinoma ( 1 ) , ovarian cancer ( 1 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 4 ) , COS (fibroblast) ( 6 , 8 ) , HCT116 (intestinal) ( 1 ) , HeLa (cervical) ( 4 ) , MEF (fibroblast) ( 1 ) , SKOV-3 (ovarian) ( 1 ) , spermatozoa ( 3 )

Upstream Regulation
Putative in vivo kinases:
GSK3B (human) ( 6 )
Kinases, in vitro:
CDK5 (human) ( 7 ) , GSK3B (human) ( 1 , 5 )
Treatments:
GSK-3_inhibitor_II ( 5 ) , mimosine ( 5 ) , nocodazole ( 5 ) , seliciclib ( 5 )

Downstream Regulation
Effects of modification on PPP1R2:
enzymatic activity, induced ( 2 ) , intracellular localization ( 8 ) , protein conformation ( 2 )
Effects of modification on biological processes:
cell cycle regulation ( 4 )
Induce interaction with:
PPP1R2 (rabbit) ( 4 )

References 

1

Xu D, et al. (2016) Obg-like ATPase 1 regulates global protein serine/threonine phosphorylation in cancer cells by suppressing the GSK3β-inhibitor 2-PP1 positive feedback loop. Oncotarget 7, 3427-39
26655089   Curated Info

2

Cannon JF (2013) How phosphorylation activates the protein phosphatase-1 • inhibitor-2 complex. Biochim Biophys Acta 1834, 71-86
22982244   Curated Info

3

Korrodi-Gregório L, et al. (2013) Identification and characterization of two distinct PPP1R2 isoforms in human spermatozoa. BMC Cell Biol 14, 15
23506001   Curated Info

4

Li M, Stukenberg PT, Brautigan DL (2008) Binding of phosphatase inhibitor-2 to prolyl isomerase Pin1 modifies specificity for mitotic phosphoproteins. Biochemistry 47, 292-300
18062707   Curated Info

5

Leach C, Shenolikar S, Brautigan DL (2003) Phosphorylation of phosphatase inhibitor-2 at centrosomes during mitosis. J Biol Chem 278, 26015-20
12697755   Curated Info

6

Sakashita G, et al. (2003) Regulation of type 1 protein phosphatase/inhibitor-2 complex by glycogen synthase kinase-3beta in intact cells. J Biochem (Tokyo) 133, 165-71
12761178   Curated Info

7

Agarwal-Mawal A, Paudel HK (2001) Neuronal Cdc2-like protein kinase (Cdk5/p25) is associated with protein phosphatase 1 and phosphorylates inhibitor-2. J Biol Chem 276, 23712-8
11320080   Curated Info

8

Kakinoki Y, Somers J, Brautigan DL (1997) Multisite phosphorylation and the nuclear localization of phosphatase inhibitor 2-green fluorescent protein fusion protein during S phase of the cell growth cycle. J Biol Chem 272, 32308-14
9405437   Curated Info