Ser847
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Home > Phosphorylation Site Page: > Ser847  -  nNOS (rat)

Site Information
SYKVRFNsVSSYSDS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447886

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 ) , mutation of modification site ( 4 , 9 ) , phospho-antibody ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 ) , western blotting ( 2 , 3 , 4 , 7 , 8 , 9 )
Disease tissue studied:
pituitary cancer ( 4 )
Relevant cell line - cell type - tissue:
'brain, hippocampus' ( 8 ) , 'neuron, cortical'-brain ( 2 , 6 ) , 'neuron, hippocampal'-'brain, embryonic' ( 2 ) , 'neuron, hippocampal'-brain ( 6 ) , 293 (epithelial) ( 4 , 7 ) , kidney ( 5 ) , muscle ( 1 ) , neuron-'brain, cerebellum' ( 4 ) , neuron-'brain, hippocampus' ( 4 ) , neuron-'brain, hypothalamus' ( 3 ) , NG108-15 (neuron) ( 9 ) , stomach ( 1 )

Upstream Regulation
Regulatory protein:
MEK1 (human) ( 4 ) , PSD-95 (human) ( 7 )
Putative in vivo kinases:
CAMK2A (rat) ( 6 , 7 , 8 , 9 ) , p90RSK (rat) ( 4 ) , RSK2 (rat) ( 4 )
Kinases, in vitro:
CAMK2A (rat) ( 6 , 9 ) , p90RSK (rat) ( 4 ) , RSK2 (rat) ( 4 )
Treatments:
4-AP ( 2 ) , A23187 ( 4 , 7 ) , acadesine ( 4 ) , adrenomedullin ( 3 ) , BAPTA-AM ( 3 ) , bicuculline ( 2 ) , ciclosporin ( 3 ) , EGF ( 4 ) , FK506 ( 3 ) , glutamic acid ( 6 ) , H-89 ( 3 , 4 ) , IBMX ( 4 ) , ischemia ( 8 ) , ischemia/reperfusion ( 8 ) , KN-93 ( 7 , 8 ) , LY294002 ( 4 ) , MK801 ( 2 ) , okadaic_acid ( 3 , 9 ) , PD98059 ( 4 ) , phorbol_ester ( 4 ) , Ro31-8220 ( 4 ) , wortmannin ( 4 )

Downstream Regulation
Effects of modification on nNOS:
enzymatic activity, inhibited ( 4 , 9 )

References 

1

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

2

Ferreira JM, Burnett AL, Rameau GA (2011) Activity-dependent regulation of surface glucose transporter-3. J Neurosci 31, 1991-9
21307237   Curated Info

3

Xu Y, Krukoff TL (2007) Adrenomedullin stimulates nitric oxide production from primary rat hypothalamic neurons: roles of calcium and phosphatases. Mol Pharmacol 72, 112-20
17446268   Curated Info

4

Song T, et al. (2007) p90 RSK-1 associates with and inhibits neuronal nitric oxide synthase. Biochem J 401, 391-8
16984226   Curated Info

5

Mount PF, et al. (2006) Phosphorylation of neuronal and endothelial nitric oxide synthase in the kidney with high and low salt diets. Nephron Physiol 102, p36-50
16244499   Curated Info

6

Rameau GA, Chiu LY, Ziff EB (2004) Bidirectional regulation of neuronal nitric-oxide synthase phosphorylation at serine 847 by the N-methyl-D-aspartate receptor. J Biol Chem 279, 14307-14
14722119   Curated Info

7

Watanabe Y, et al. (2003) Post-synaptic density-95 promotes calcium/calmodulin-dependent protein kinase II-mediated Ser847 phosphorylation of neuronal nitric oxide synthase. Biochem J 372, 465-71
12630910   Curated Info

8

Osuka K, et al. (2002) Phosphorylation of neuronal nitric oxide synthase at Ser847 by CaM-KII in the hippocampus of rat brain after transient forebrain ischemia. J Cereb Blood Flow Metab 22, 1098-106
12218415   Curated Info

9

Komeima K, Hayashi Y, Naito Y, Watanabe Y (2000) Inhibition of neuronal nitric-oxide synthase by calcium/ calmodulin-dependent protein kinase IIalpha through Ser847 phosphorylation in NG108-15 neuronal cells. J Biol Chem 275, 28139-43
10874031   Curated Info

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