Ser188
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Home > Phosphorylation Site Page: > Ser188  -  RHOA (mouse)

Site Information
ARRGKKKsGCLIL__   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449035

In vivo Characterization
Methods used to characterize site in vivo:
mutation of modification site ( 1 , 4 ) , phospho-antibody ( 2 , 3 , 4 ) , western blotting ( 1 , 2 , 3 , 4 )
Disease tissue studied:
chronic myelogenous leukemia ( 1 ) , liver cancer ( 1 ) , hepatocellular carcinoma ( 1 )
Relevant cell line - cell type - tissue:
'muscle, smooth'-thoracic aorta ( 4 ) , 'muscle, smooth'-thoracic aorta [RHOA (mouse), heterozygous knockout] ( 4 ) , C2C12 (myoblast) ( 3 ) , HAP1 ( 1 ) , Huh7 (hepatic) ( 1 ) , LLC-PK1 (renal) ( 2 )

Upstream Regulation
Putative in vivo kinases:
ChaK1 (human) ( 1 ) , PKG1 (mouse) ( 4 )
Kinases, in vitro:
ChaK1 (human) ( 1 )
Treatments:
cGMP_analog ( 4 ) , compression ( 3 ) , DT-3 ( 4 ) , H-89 ( 3 ) , Mn(2+) ( 2 ) , NS8593 ( 1 ) , Rp-8pCPT-cGMPS ( 4 ) , SQ22536 ( 3 ) , vasopressin ( 2 )

Downstream Regulation
Effects of modification on RHOA:
activity, inhibited ( 5 )
Effects of modification on biological processes:
transcription, altered ( 4 ) , transcription, inhibited ( 5 )

References 

1

Voringer S, et al. (2019) Inhibition of TRPM7 blocks MRTF/SRF-dependent transcriptional and tumorigenic activity. Oncogene
31844251   Curated Info

2

Lei L, et al. (2017) Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. Am J Physiol Renal Physiol, ajprenal003912017
29046300   Curated Info

3

Takemoto K, et al. (2015) Compressive stress induces dephosphorylation of the myosin regulatory light chain via RhoA phosphorylation by the adenylyl cyclase/protein kinase A signaling pathway. PLoS One 10, e0117937
25734240   Curated Info

4

Sawada N, et al. (2009) Cyclic GMP kinase and RhoA Ser188 phosphorylation integrate pro- and antifibrotic signals in blood vessels. Mol Cell Biol 29, 6018-32
19737918   Curated Info

5

Ling L, Lobie PE (2004) RhoA/ROCK activation by growth hormone abrogates p300/histone deacetylase 6 repression of Stat5-mediated transcription. J Biol Chem 279, 32737-50
15102857   Curated Info