Lys689
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.0.1
Powered by Cell Signaling Technology
Home > Sumoylation Site Page: > Lys689  -  CTCF (human)

Site Information
ENIIVEVkKEPDAEP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 6124704

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 4 ) , immunoprecipitation ( 4 ) , mass spectrometry ( 2 , 3 ) , modification-specific antibody ( 4 ) , mutation of modification site ( 4 ) , western blotting ( 4 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 4 ) , 293N3S (epithelial) ( 3 ) , alpha-TC6 (pancreatic) ( 4 ) , HeLa (cervical) [SUMO2 (human), transfection, SUMO2 K0 Q87R (lysine-deficient SUMO2, with Q87R to create convenient Trypsin cleavage site)] ( 2 ) , hTCEpi (epithelial) ( 4 )

Upstream Regulation
Regulatory protein:
SENP3 (human) ( 4 ) , SENP5 (human) ( 4 )
Treatments:
H2O2 ( 4 ) , heat_shock ( 4 ) , hyperosmotic shock ( 4 ) , hypoxia ( 4 ) , siRNA ( 4 ) , UV ( 4 )

Downstream Regulation
Effects of modification on biological processes:
transcription, induced ( 4 )

References 

1

Lumpkin RJ, et al. (2017) Site-specific identification and quantitation of endogenous SUMO modifications under native conditions. Nat Commun 8, 1171
29079793   Curated Info

2

Hendriks IA, et al. (2014) Uncovering global SUMOylation signaling networks in a site-specific manner. Nat Struct Mol Biol 21, 927-36
25218447   Curated Info

3

Tammsalu T, et al. (2014) Proteome-wide identification of SUMO2 modification sites. Sci Signal 7, rs2
24782567   Curated Info

4

Wang J, Wang Y, Lu L (2012) De-SUMOylation of CCCTC binding factor (CTCF) in hypoxic stress-induced human corneal epithelial cells. J Biol Chem 287, 12469-79
22354964   Curated Info

Developed with grants from  NIH Logo and literature mining with Linguamatics  I2E Logo Creative Commons License PhosphoSite, created by Cell Signaling Technology is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.

©2003-2019 Cell Signaling Technology, Inc.