Ser170
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Home > Phosphorylation Site Page: > Ser170  -  NFAT3 (human)

Site Information
GGAFFsPsPGsSSLS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448860

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 3 ) , electrophoretic mobility shift ( 3 ) , mass spectrometry ( 1 ) , mutation of modification site ( 3 ) , phospho-antibody ( 2 ) , phosphopeptide mapping ( 3 ) , western blotting ( 2 )
Relevant cell line - cell type - tissue:
293T (epithelial) ( 3 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 3 ) , BHK-21 (fibroblast) ( 3 ) , C2C12 (myoblast) ( 2 ) , COS (fibroblast) ( 3 ) , Jurkat (T lymphocyte) ( 1 )

Upstream Regulation
Putative in vivo kinases:
P38A (human) ( 3 )
Treatments:
ionomycin ( 3 ) , UV ( 3 )

Downstream Regulation
Effects of modification on NFAT3:
activity, inhibited ( 3 ) , intracellular localization ( 3 )
Effects of modification on biological processes:
cell differentiation, altered ( 2 ) , cell growth, altered ( 3 ) , transcription, altered ( 3 )

References 

1

Zhou J (2011) CST Curation Set: 12497; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

2

Cho YY, et al. (2007) RSK2 mediates muscle cell differentiation through regulation of NFAT3. J Biol Chem 282, 8380-92
17213202   Curated Info

3

Yang TT, et al. (2002) Phosphorylation of NFATc4 by p38 mitogen-activated protein kinases. Mol Cell Biol 22, 3892-904
11997522   Curated Info