Thr357
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Home > Phosphorylation Site Page: > Thr357  -  MLKL (human)

Site Information
FELRkTQtsMSLGTt   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 5891858

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 7 ) , immunoassay ( 6 ) , immunoprecipitation ( 4 , 7 ) , mass spectrometry ( 7 , 8 ) , mutation of modification site ( 6 , 7 ) , phospho-antibody ( 3 , 4 , 5 , 6 ) , western blotting ( 4 , 5 , 6 )
Disease tissue studied:
Alzheimer's disease ( 3 ) , colorectal cancer ( 4 , 6 , 7 ) , colorectal carcinoma ( 4 , 6 , 7 ) , lymphoma ( 6 )
Relevant cell line - cell type - tissue:
brain ( 3 , 5 ) , HeLa (cervical) ( 6 , 7 ) , HT-29 (intestinal) ( 4 , 6 , 7 ) , Jurkat (T lymphocyte) ( 8 ) , liver ( 6 ) , U-937 (myeloid) ( 6 )

Upstream Regulation
Putative in vivo kinases:
RIPK3 (human) ( 6 , 7 )
Kinases, in vitro:
RIPK3 (human) ( 7 )
Treatments:
LPS ( 6 ) , Necrostatin-1 ( 6 ) , TNF ( 6 ) , Z-VAD-FMK ( 6 )

Downstream Regulation
Effects of modification on MLKL:
intracellular localization ( 3 , 4 , 6 ) , molecular association, regulation ( 6 ) , protein conformation ( 3 , 4 )
Effects of modification on biological processes:
apoptosis, induced ( 6 ) , signaling pathway regulation ( 7 )
Induce interaction with:
MLKL (human) ( 6 )

Disease / Diagnostics Relevance
Relevant diseases:
Alzheimer's disease ( 3 ) , multiple sclerosis ( 5 )

References 

1

Chen X, et al. (2022) Mosaic composition of RIP1-RIP3 signalling hub and its role in regulating cell death. Nat Cell Biol
35256774   Curated Info

2

Zu R, et al. (2021) Quantitative analysis of phosphoproteome in necroptosis reveals a role of TRIM28 phosphorylation in promoting necroptosis-induced cytokine production. Cell Death Dis 12, 994
34689152   Curated Info

3

Caccamo A, et al. (2017) Necroptosis activation in Alzheimer's disease. Nat Neurosci 20, 1236-1246
28758999   Curated Info

4

Yoon S, Bogdanov K, Kovalenko A, Wallach D (2016) Necroptosis is preceded by nuclear translocation of the signaling proteins that induce it. Cell Death Differ 23, 253-60
26184911   Curated Info

5

Ofengeim D, et al. (2015) Activation of necroptosis in multiple sclerosis. Cell Rep 10, 1836-49
25801023   Curated Info

6

Wang H, et al. (2014) Mixed Lineage Kinase Domain-like Protein MLKL Causes Necrotic Membrane Disruption upon Phosphorylation by RIP3. Mol Cell 54, 133-46
24703947   Curated Info

7

Sun L, et al. (2012) Mixed lineage kinase domain-like protein mediates necrosis signaling downstream of RIP3 kinase. Cell 148, 213-27
22265413   Curated Info

8

Moritz A (2007) CST Curation Set: 2970; Year: 2007; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info