Thr305
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Home > Phosphorylation Site Page: > Thr305  -  CAMK2A (rat)

Site Information
kLkGAILttMLAtRN   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448802

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 8 ) , mass spectrometry ( 4 ) , mutation of modification site ( 1 , 2 ) , phospho-antibody ( 3 ) , phosphopeptide mapping ( 8 ) , western blotting ( 3 )
Relevant cell line - cell type - tissue:
'brain, hippocampus' ( 3 , 8 ) , brain ( 4 ) , neuron-'brain, hippocampus' ( 1 , 2 )

Upstream Regulation
Kinases, in vitro:
CAMK2A (human) ( 7 ) , CAMK2A (rat) ( 5 , 6 , 9 , 10 )
Treatments:
ischemia ( 3 )

Downstream Regulation
Effects of modification on CAMK2A:
enzymatic activity, inhibited ( 7 , 10 ) , molecular association, regulation ( 7 , 10 )
Effects of modification on biological processes:
cytoskeletal reorganization ( 1 )
Inhibit interaction with:
Calmodulin (cow) ( 10 ) , Calmodulin (rat) ( 7 )

References 

1

Pi HJ, et al. (2010) CaMKII control of spine size and synaptic strength: role of phosphorylation states and nonenzymatic action. Proc Natl Acad Sci U S A 107, 14437-42
20660727   Curated Info

2

Pi HJ, et al. (2010) Autonomous CaMKII can promote either long-term potentiation or long-term depression, depending on the state of T305/T306 phosphorylation. J Neurosci 30, 8704-9
20592192   Curated Info

3

Davies KD, Alvestad RM, Coultrap SJ, Browning MD (2007) alphaCaMKII autophosphorylation levels differ depending on subcellular localization. Brain Res 1158, 39-49
17559813   Curated Info

4

Guo A (2007) CST Curation Set: 2239; Year: 2007; Biosample/Treatment: tissue, brain/ischemia 20'; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]Q Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

5

Takao K, et al. (2005) Visualization of synaptic Ca2+ /calmodulin-dependent protein kinase II activity in living neurons. J Neurosci 25, 3107-12
15788767   Curated Info

6

Ishida A, Fujisawa H (1995) Stabilization of calmodulin-dependent protein kinase II through the autoinhibitory domain. J Biol Chem 270, 2163-70
7836445   Curated Info

7

Hanson PI, Schulman H (1992) Inhibitory autophosphorylation of multifunctional Ca2+/calmodulin-dependent protein kinase analyzed by site-directed mutagenesis. J Biol Chem 267, 17216-24
1324926   Curated Info

8

Molloy SS, Kennedy MB (1991) Autophosphorylation of type II Ca2+/calmodulin-dependent protein kinase in cultures of postnatal rat hippocampal slices. Proc Natl Acad Sci U S A 88, 4756-60
1647015   Curated Info

9

Patton BL, Miller SG, Kennedy MB (1990) Activation of type II calcium/calmodulin-dependent protein kinase by Ca2+/calmodulin is inhibited by autophosphorylation of threonine within the calmodulin-binding domain. J Biol Chem 265, 11204-12
2162838   Curated Info

10

Colbran RJ, Soderling TR (1990) Calcium/calmodulin-independent autophosphorylation sites of calcium/calmodulin-dependent protein kinase II. Studies on the effect of phosphorylation of threonine 305/306 and serine 314 on calmodulin binding using synthetic peptides. J Biol Chem 265, 11213-9
2162839   Curated Info