Ser152
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Home > Phosphorylation Site Page: > Ser152  -  p16-INK4A (human)

Site Information
IDAAEGPsDIPD___   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448832

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 4 ) , immunoprecipitation ( 4 ) , mass spectrometry ( 1 ) , mutation of modification site ( 3 , 4 ) , peptide sequencing ( 4 ) , phospho-antibody ( 2 ) , phosphoamino acid analysis ( 4 ) , western blotting ( 2 )
Disease tissue studied:
bone cancer ( 3 ) , breast cancer ( 1 ) , breast cancer, triple negative ( 1 )
Relevant cell line - cell type - tissue:
breast ( 1 ) , HFSN1 (fibroblast) ( 2 ) , U2OS (bone cell) [GR (human)] ( 4 ) , U2OS (bone cell) ( 3 ) , WI-38 (fibroblast) ( 3 , 4 )

Upstream Regulation
Putative in vivo kinases:
ATR (human) ( 2 )
Treatments:
siRNA ( 2 ) , UV ( 2 )

Downstream Regulation
Effects of modification on p16-INK4A:
molecular association, regulation ( 2 ) , protein stabilization ( 2 )
Induce interaction with:
CDK4 (human) ( 2 )

References 

1

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

2

Al-Khalaf HH, Hendrayani SF, Aboussekhra A (2011) The atr protein kinase controls UV-dependent upregulation of p16INK4A through inhibition of Skp2-related polyubiquitination/degradation. Mol Cancer Res 9, 311-9
21270107   Curated Info

3

Guo Y, Yuan C, Weghorst CM, Li J (2010) IKKbeta specifically binds to P16 and phosphorylates Ser8 of P16. Biochem Biophys Res Commun 393, 504-8
20152798   Curated Info

4

Gump J, Stokoe D, McCormick F (2003) Phosphorylation of p16INK4A correlates with Cdk4 association. J Biol Chem 278, 6619-22
12529334   Curated Info