Ser140
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Home > Phosphorylation Site Page: > Ser140  -  p16-INK4A (human)

Site Information
AAGGTrGsNHARIDA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448831

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 4 ) , flow cytometry ( 1 ) , immunoprecipitation ( 1 , 4 ) , mutation of modification site ( 1 , 3 , 4 ) , peptide sequencing ( 4 ) , phospho-antibody ( 1 , 2 ) , phosphoamino acid analysis ( 4 ) , western blotting ( 1 , 2 )
Disease tissue studied:
bone cancer ( 3 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
ATR (human) ( 2 )
Treatments:
H2O2 ( 1 ) , siRNA ( 2 ) , UV ( 2 )

Downstream Regulation
Effects of modification on p16-INK4A:
molecular association, regulation ( 1 , 2 ) , protein stabilization ( 2 )
Effects of modification on biological processes:
apoptosis, induced ( 1 ) , cell growth, inhibited ( 1 )
Induce interaction with:
CDK4 (human) ( 1 , 2 )

References 

1

Lu Y, et al. (2017) The interplay between p16 serine phosphorylation and arginine methylation determines its function in modulating cellular apoptosis and senescence. Sci Rep 7, 41390
28120917   Curated Info

2

Al-Khalaf HH, Hendrayani SF, Aboussekhra A (2011) The atr protein kinase controls UV-dependent upregulation of p16INK4A through inhibition of Skp2-related polyubiquitination/degradation. Mol Cancer Res 9, 311-9
21270107   Curated Info

3

Guo Y, Yuan C, Weghorst CM, Li J (2010) IKKbeta specifically binds to P16 and phosphorylates Ser8 of P16. Biochem Biophys Res Commun 393, 504-8
20152798   Curated Info

4

Gump J, Stokoe D, McCormick F (2003) Phosphorylation of p16INK4A correlates with Cdk4 association. J Biol Chem 278, 6619-22
12529334   Curated Info