Thr165

Site Information
FACTYVGtPYYVPPE SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 57829902

In vivo Characterization
Methods used to characterize site in vivo:	immunoprecipitation ( 1 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Disease tissue studied:	breast cancer ( 1 )
Relevant cell line - cell type - tissue:	HEK293T (epithelial) ( 1 ) , MCF-7 (breast cell) ( 1 ) , T47D (breast cell) ( 1 )

Downstream Regulation
Effects of modification on NEK3:	enzymatic activity, induced ( 1 )
Effects of modification on biological processes:	cell adhesion, altered ( 1 ) , cell motility, induced ( 1 )

References
1	Harrington KM, Clevenger CV (2016) Identification of NEK3 Kinase Threonine 165 as a Novel Regulatory Phosphorylation Site That Modulates Focal Adhesion Remodeling Necessary for Breast Cancer Cell Migration. J Biol Chem 291, 21388-21406 27489110 Curated Info