Ser332
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Home > Phosphorylation Site Page: > Ser332  -  C5aR (human)

Site Information
EEsVVREsKsFTRsT   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448822

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 5 , 6 ) , mass spectrometry ( 1 , 2 ) , mutation of modification site ( 3 , 4 , 5 , 6 ) , phosphopeptide mapping ( 6 ) , western blotting ( 4 , 5 )
Disease tissue studied:
HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , lung cancer ( 2 ) , non-small cell lung adenocarcinoma ( 2 )
Relevant cell line - cell type - tissue:
breast ( 1 ) , COS (fibroblast) ( 5 , 6 ) , HL60 (myeloid) ( 5 , 6 ) , lung ( 2 ) , RBL-2H3 (basophil) ( 3 ) , RIN (epithelial) ( 4 )

Upstream Regulation
Kinases, in vitro:
PKCA (human) ( 6 )
Treatments:
C5a ( 5 , 6 ) , phorbol_ester ( 6 )

Downstream Regulation
Effects of modification on C5aR:
molecular association, regulation ( 4 ) , receptor desensitization, altered ( 3 , 5 )
Induce interaction with:
ARRB1 (human) ( 4 ) , ARRB2 (human) ( 4 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
23911959   Curated Info

3

Pollok-Kopp B, H├╝ttenrauch F, Rethorn S, Oppermann M (2007) Dynamics of protein kinase C-mediated phosphorylation of the complement C5a receptor on serine 334. J Biol Chem 282, 4345-53
17145764   Curated Info

4

Braun L, Christophe T, Boulay F (2003) Phosphorylation of key serine residues is required for internalization of the complement 5a (C5a) anaphylatoxin receptor via a beta-arrestin, dynamin, and clathrin-dependent pathway. J Biol Chem 278, 4277-85
12464600   Curated Info

5

Christophe T, et al. (2000) Human complement 5a (C5a) anaphylatoxin receptor (CD88) phosphorylation sites and their specific role in receptor phosphorylation and attenuation of G protein-mediated responses. Desensitization of C5a receptor controls superoxide production but not receptor sequestration in HL-60 cells. J Biol Chem 275, 1656-64
10636859   Curated Info

6

Giannini E, Brouchon L, Boulay F (1995) Identification of the major phosphorylation sites in human C5a anaphylatoxin receptor in vivo. J Biol Chem 270, 19166-72
7642584   Curated Info