Ser126
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Home > Phosphorylation Site Page: > Ser126  -  CCNB1 (human)

Site Information
PILVDTAsPsPMETs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448814

In vivo Characterization
Methods used to characterize site in vivo:
flow cytometry ( 4 ) , immunoassay ( 3 ) , immunoprecipitation ( 10 ) , mass spectrometry ( 1 , 10 ) , mutation of modification site ( 6 , 7 , 8 , 9 , 10 ) , phospho-antibody ( 1 , 2 , 4 , 5 , 6 , 8 ) , western blotting ( 1 , 4 , 5 , 8 , 9 )
Disease tissue studied:
bone cancer ( 4 , 8 ) , vulvar cancer ( 2 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 1 ) , COS (fibroblast) ( 9 ) , HeLa (cervical) ( 1 , 6 , 7 , 8 , 9 , 10 ) , hTERT-RPE1 (epithelial) ( 3 , 4 ) , MEF (fibroblast) [MASTL (mouse), heterozygous knockout] ( 5 ) , U2OS (bone cell) ( 4 , 8 )

Upstream Regulation
Regulatory protein:
MASTL (human) ( 5 ) , PPP2CA (human) ( 5 )
Putative in vivo kinases:
CDK1 (human) ( 4 ) , PLK1 (human) ( 10 )
Kinases, in vitro:
CDK1 (human) ( 9 ) , ERK2 (human) ( 9 ) , PLK1 (human) ( 10 )
Treatments:
ionizing_radiation ( 3 ) , RO-3306 ( 4 )

Downstream Regulation
Effects of modification on CCNB1:
intracellular localization ( 2 , 6 , 7 )
Effects of modification on biological processes:
carcinogenesis, induced ( 2 ) , cell cycle regulation ( 3 , 4 )
Induce interaction with:
DNA ( 6 )

Disease / Diagnostics Relevance
Relevant diseases:
vulvar cancer ( 2 )

References 

1

Gheghiani L, et al. (2017) PLK1 Activation in Late G2 Sets Up Commitment to Mitosis. Cell Rep 19, 2060-2073
28591578   Curated Info

2

Wang Z, et al. (2015) Expression of CDK1Tyr15, pCDK1Thr161, Cyclin B1 (Total) and pCyclin B1Ser126 in Vulvar Squamous Cell Carcinoma and Their Relations with Clinicopatological Features and Prognosis. PLoS One 10, e0121398
25849598   Curated Info

3

Krenning L, et al. (2014) Transient Activation of p53 in G2 Phase Is Sufficient to Induce Senescence. Mol Cell 55, 59-72
24910099   Curated Info

4

Akopyan K, et al. (2014) Assessing kinetics from fixed cells reveals activation of the mitotic entry network at the s/g2 transition. Mol Cell 53, 843-53
24582498   Curated Info

5

Álvarez-Fernández M, et al. (2013) Greatwall is essential to prevent mitotic collapse after nuclear envelope breakdown in mammals. Proc Natl Acad Sci U S A 110, 17374-9
24101512   Curated Info

6

Santos SD, Wollman R, Meyer T, Ferrell JE (2012) Spatial positive feedback at the onset of mitosis. Cell 149, 1500-13
22726437   Curated Info

7

Jang CY, Coppinger JA, Yates JR, Fang G (2010) Phospho-regulation of DDA3 function in mitosis. Biochem Biophys Res Commun 393, 259-63
20117088   Curated Info

8

Jackman M, Lindon C, Nigg EA, Pines J (2003) Active cyclin B1-Cdk1 first appears on centrosomes in prophase. Nat Cell Biol 5, 143-8
12524548   Curated Info

9

Yuan J, et al. (2002) Cooperative phosphorylation including the activity of polo-like kinase 1 regulates the subcellular localization of cyclin B1. Oncogene 21, 8282-92
12447691   Curated Info

10

Toyoshima-Morimoto F, et al. (2001) Polo-like kinase 1 phosphorylates cyclin B1 and targets it to the nucleus during prophase. Nature 410, 215-20
11242082   Curated Info