Tyr228

Site Information
EKIWMTGyEDyRHAP SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 471403

In vivo Characterization
Methods used to characterize site in vivo:	immunoprecipitation ( 2 ) , mass spectrometry ( 1 ) , mutation of modification site ( 2 ) , western blotting ( 2 )
Disease tissue studied:	neuroblastoma ( 1 )
Relevant cell line - cell type - tissue:	HEK293T (epithelial) ( 2 ) , SK-N-BE(2) (neural crest) ( 1 )

Upstream Regulation
Regulatory protein:	EGFR (human) ( 2 )
Treatments:	EGF ( 2 ) , vanadate ( 2 )

Downstream Regulation
Effects of modification on SPRED2:	protein degradation ( 2 )

References
1	Guo A (2010) CST Curation Set: 10549; Year: 2010; Biosample/Treatment: cell line, SKNBE(2)/NGF; Disease: neuroblastoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
2	Lock P, et al. (2006) Spred-2 steady-state levels are regulated by phosphorylation and Cbl-mediated ubiquitination. Biochem Biophys Res Commun 351, 1018-23 17094949 Curated Info

References

Guo A (2010) CST Curation Set: 10549; Year: 2010; Biosample/Treatment: cell line, SKNBE(2)/NGF; Disease: neuroblastoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

Lock P, et al. (2006) Spred-2 steady-state levels are regulated by phosphorylation and Cbl-mediated ubiquitination. Biochem Biophys Res Commun 351, 1018-23
17094949 Curated Info