Ser8

Site Information
MGTVLSLsPSYRKAT SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 471392

In vivo Characterization
Methods used to characterize site in vivo:	2D analysis ( 2 ) , [32P] bio-synthetic labeling ( 2 ) , electrophoretic mobility shift ( 1 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 , 2 ) , western blotting ( 1 )
Relevant cell line - cell type - tissue:	'neuron, cortical'-brain ( 1 , 2 ) , COS (fibroblast) ( 1 ) , HeLa (cervical) ( 2 )

Upstream Regulation
Putative in vivo kinases:	CDK5 (human) ( 1 )

References
1	Hosokawa T, et al. (2010) Quantitative measurement of in vivo phosphorylation states of Cdk5 activator p35 by Phos-tag SDS-PAGE. Mol Cell Proteomics 9, 1133-43 20097924 Curated Info
2	Kamei H, et al. (2007) Suppression of calpain-dependent cleavage of the CDK5 activator p35 to p25 by site-specific phosphorylation. J Biol Chem 282, 1687-94 17121855 Curated Info