Thr138

Site Information
PAVTSAGtPKRVIVQ SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 471393

In vivo Characterization
Methods used to characterize site in vivo:	2D analysis ( 7 ) , immunoprecipitation ( 2 , 4 , 6 ) , mass spectrometry ( 1 ) , mutation of modification site ( 3 , 4 , 5 , 6 , 7 ) , phospho-antibody ( 3 , 4 ) , western blotting ( 2 , 3 , 4 )
Disease tissue studied:	adrenal cancer ( 5 , 6 ) , pheochromocytoma ( 5 , 6 ) , neuroblastoma ( 1 , 2 )
Relevant cell line - cell type - tissue:	293 (epithelial) ( 2 , 3 , 4 , 7 ) , CLB-Bar ( 1 ) , COS (fibroblast) ( 3 , 7 ) , COS7 (fibroblast) ( 4 ) , HEK293T (epithelial) ( 6 ) , Neuro-2a (neuron) ( 2 ) , PC-12 (chromaffin) ( 5 , 6 )

Downstream Regulation
Effects of modification on CDK5R1:	activity, inhibited ( 6 ) , molecular association, regulation ( 4 ) , protein conformation ( 4 ) , protein degradation ( 2 ) , protein stabilization ( 7 )
Effects of modification on biological processes:	cell growth, altered ( 6 ) , cytoskeletal reorganization ( 4 , 6 )

References
1	Borenäs M, et al. (2024) ALK signaling primes the DNA damage response sensitizing ALK-driven neuroblastoma to therapeutic ATR inhibition. Proc Natl Acad Sci U S A 121, e2315242121 38154064 Curated Info
2	Takasugi T, et al. (2016) Two Degradation Pathways of the p35 Cdk5 (Cyclin-dependent Kinase) Activation Subunit, Dependent and Independent of Ubiquitination. J Biol Chem 291, 4649-57 26631721 Curated Info
3	Asada A, Saito T, Hisanaga S (2012) Phosphorylation of p35 and p39 by Cdk5 determines the subcellular location of the holokinase in a phosphorylation-site-specific manner. J Cell Sci 125, 3421-9 22467861 Curated Info
4	He L, et al. (2011) The neuronal p35 activator of Cdk5 is a novel F-actin binding and bundling protein. Cell Mol Life Sci 68, 1633-43 20976519 Curated Info
5	Son MY, Chung SH (2010) Expression of p25, an aberrant cyclin-dependent kinase 5 activator, stimulates basal secretion in PC12 cells. Mol Cells 29, 51-6 20033852 Curated Info
6	He L, Hou Z, Qi RZ (2008) Calmodulin binding and Cdk5 phosphorylation of p35 regulate its effect on microtubules. J Biol Chem 283, 13252-60 18326489 Curated Info
7	Kamei H, et al. (2007) Suppression of calpain-dependent cleavage of the CDK5 activator p35 to p25 by site-specific phosphorylation. J Biol Chem 282, 1687-94 17121855 Curated Info