Tyr305
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Home > Phosphorylation Site Page: > Tyr305  -  IkB-alpha (human)

Site Information
FtEDELPyDDCVFGG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448799

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 8 ) , immunoprecipitation ( 8 ) , mass spectrometry ( 2 , 3 , 4 , 5 , 6 ) , mutation of modification site ( 1 , 7 , 8 ) , phospho-antibody ( 1 , 7 , 8 ) , western blotting ( 1 , 8 )
Disease tissue studied:
bone cancer ( 8 ) , chronic lymphocytic leukemia ( 1 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
Bcr (human) ( 1 )
Putative in vivo kinases:
Abl (human) ( 8 ) , Btk (human) ( 1 )
Kinases, in vitro:
Btk (human) ( 1 )

Downstream Regulation
Effects of modification on IkB-alpha:
activity, inhibited ( 1 ) , intracellular localization ( 8 ) , protein degradation ( 7 ) , protein stabilization ( 8 )
Effects of modification on biological processes:
apoptosis, induced ( 8 ) , signaling pathway regulation ( 1 ) , transcription, inhibited ( 8 )

Disease / Diagnostics Relevance
Relevant diseases:
chronic lymphocytic leukemia ( 1 )

References 

1

Pontoriero M, et al. (2019) Activation of NF-κB in B cell receptor signaling through Bruton's tyrosine kinase-dependent phosphorylation of IκB-α. J Mol Med (Berl)
30887112   Curated Info

2

Zhou J (2012) CST Curation Set: 12611; Year: 2012; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

3

Zhou J (2012) CST Curation Set: 12612; Year: 2012; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY
Curated Info

4

Zhou J (2011) CST Curation Set: 12495; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Zhou J (2011) CST Curation Set: 12496; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Zhou J (2011) CST Curation Set: 12497; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

Waris G, et al. (2003) Hepatitis C virus NS5A and subgenomic replicon activate NF-kappaB via tyrosine phosphorylation of IkappaBalpha and its degradation by calpain protease. J Biol Chem 278, 40778-87
12909638   Curated Info

8

Kawai H, Nie L, Yuan ZM (2002) Inactivation of NF-kappaB-dependent cell survival, a novel mechanism for the proapoptotic function of c-Abl. Mol Cell Biol 22, 6079-88
12167702   Curated Info