Ser16

Site Information
AGGPRPEsPVPAGRA SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 471220

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 17 , 18 , 19 , 20 , 21 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 )
Disease tissue studied:	breast cancer ( 8 , 9 , 19 , 20 ) , breast ductal carcinoma ( 8 ) , HER2 positive breast cancer ( 3 ) , luminal A breast cancer ( 3 ) , luminal B breast cancer ( 3 ) , breast cancer, surrounding tissue ( 3 ) , breast cancer, triple negative ( 3 , 8 ) , cervical cancer ( 32 ) , cervical adenocarcinoma ( 32 ) , leukemia ( 23 ) , acute myelogenous leukemia ( 23 ) , lung cancer ( 13 , 20 , 35 ) , non-small cell lung cancer ( 20 , 35 ) , non-small cell lung adenocarcinoma ( 13 ) , neuroblastoma ( 18 ) , ovarian cancer ( 8 ) , pancreatic ductal adenocarcinoma ( 12 ) , melanoma skin cancer ( 6 )
Relevant cell line - cell type - tissue:	'pancreatic, ductal'-pancreas ( 12 ) , 293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] ( 29 ) , 293 (epithelial) [AT1 (human), transfection] ( 28 ) , 786-O (renal) [VHL (human), transfection] ( 5 ) , 786-O (renal) ( 5 ) , A498 (renal) ( 31 ) , A549 (pulmonary) ( 14 ) , breast ( 3 , 8 ) , BT-20 (breast cell) ( 20 ) , BT-549 (breast cell) ( 20 ) , Calu 6 (pulmonary) ( 20 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 21 ) , Flp-In T-Rex-293 (epithelial) ( 21 ) , H2009 (pulmonary) ( 20 ) , H2077 (pulmonary) ( 20 ) , H2887 (pulmonary) ( 20 ) , H322M (pulmonary) ( 20 ) , HCC1359 (pulmonary) ( 20 ) , HCC1937 (breast cell) ( 20 ) , HCC366 (pulmonary) ( 20 ) , HCC4006 (pulmonary) ( 20 ) , HCC78 (pulmonary) ( 20 ) , HCT116 (intestinal) ( 34 ) , HeLa (cervical) ( 2 , 7 , 17 , 24 , 27 , 33 , 37 , 39 , 41 ) , HeLa S3 (cervical) ( 25 , 32 , 36 ) , HeLa_Meta (cervical) ( 30 ) , HeLa_Pro (cervical) ( 30 ) , HeLa_Telo (cervical) ( 30 ) , HMLER ('stem, breast cancer') [CXCR4 (human), knockdown] ( 9 ) , HMLER ('stem, breast cancer') ( 9 ) , HOP62 (pulmonary) ( 20 ) , HUES-9 ('stem, embryonic') ( 26 ) , Jurkat (T lymphocyte) ( 15 ) , K562 (erythroid) ( 17 , 33 ) , KG-1 (myeloid) ( 23 ) , liver ( 11 ) , lung ( 13 ) , MCF-7 (breast cell) ( 20 ) , MDA-MB-231 (breast cell) ( 20 ) , MDA-MB-468 (breast cell) ( 20 ) , MV4-11 (macrophage) ( 34 ) , NB10 (neural crest) ( 18 ) , NCI-H1299 (pulmonary) ( 35 ) , NCI-H1395 (pulmonary) ( 20 ) , NCI-H1568 (pulmonary) ( 20 ) , NCI-H157 (pulmonary) ( 20 ) , NCI-H1648 (pulmonary) ( 20 ) , NCI-H1666 (pulmonary) ( 20 ) , NCI-H2030 (pulmonary) ( 20 ) , NCI-H322 (pulmonary) ( 20 ) , NCI-H520 (squamous) ( 20 ) , NCI-H647 (pulmonary) ( 20 ) , NPC (neural crest) ( 18 ) , ovary ( 8 ) , PC9 (pulmonary) ( 20 ) , platelet-blood ( 40 ) , SH-SY5Y (neural crest) ( 10 ) , SKBr3 (breast cell) ( 19 ) , TERT20 ('stem, mesenchymal') ( 38 ) , Vero E6-S ('epithelial, kidney') ( 1 ) , WM239A (melanocyte) ( 6 )

Upstream Regulation
Treatments:	LRRK2-IN-1 ( 10 ) , MG132_withdrawal ( 30 ) , nocodazole ( 32 )

References
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2	Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296 27281782 Curated Info
3	Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62 27251275 Curated Info
4	Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610 27184836 Curated Info
5	Malec V, Coulson JM, Urbé S, Clague MJ (2015) Combined Analyses of the VHL and Hypoxia Signaling Axes in an Isogenic Pairing of Renal Clear Cell Carcinoma Cells. J Proteome Res 14, 5263-72 26506913 Curated Info
6	Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615 25850435 Curated Info
7	Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94 25159151 Curated Info
8	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
9	Yi T, et al. (2014) Quantitative phosphoproteomic analysis reveals system-wide signaling pathways downstream of SDF-1/CXCR4 in breast cancer stem cells. Proc Natl Acad Sci U S A 111, E2182-90 24782546 Curated Info
10	Luerman GC, et al. (2014) Phosphoproteomic evaluation of pharmacological inhibition of leucine-rich repeat kinase 2 reveals significant off-target effects of LRRK-2-IN-1. J Neurochem 128, 561-76 24117733 Curated Info
11	Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62 24275569 Curated Info
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14	Kim JY, et al. (2013) Dissection of TBK1 signaling via phosphoproteomics in lung cancer cells. Proc Natl Acad Sci U S A 110, 12414-9 23836654 Curated Info
15	Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7 23749302 Curated Info
16	Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21 23312004 Curated Info
17	Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71 23186163 Curated Info
18	DeNardo BD, et al. (2013) Quantitative phosphoproteomic analysis identifies activation of the RET and IGF-1R/IR signaling pathways in neuroblastoma. PLoS One 8, e82513 24349301 Curated Info
19	Imami K, et al. (2012) Temporal profiling of lapatinib-suppressed phosphorylation signals in EGFR/HER2 pathways. Mol Cell Proteomics 11, 1741-57 22964224 Curated Info
20	Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68 22617229 Curated Info
21	Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70 22496350 Curated Info
22	Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25 22424773 Curated Info
23	Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56 22115753 Curated Info
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25	Santamaria A, et al. (2011) The Plk1-dependent phosphoproteome of the early mitotic spindle. Mol Cell Proteomics 10, M110.004457 20860994 Curated Info
26	Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3 21406692 Curated Info
27	Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5 21712546 Curated Info
28	Xiao K, et al. (2010) Global phosphorylation analysis of beta-arrestin-mediated signaling downstream of a seven transmembrane receptor (7TMR). Proc Natl Acad Sci U S A 107, 15299-304 20686112 Curated Info
29	Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53 20363803 Curated Info
30	Dulla K, et al. (2010) Quantitative site-specific phosphorylation dynamics of human protein kinases during mitotic progression. Mol Cell Proteomics 9, 1167-81 20097925 Curated Info
31	Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62 20071362 Curated Info
32	Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3 20068231 Curated Info
33	Pan C, Olsen JV, Daub H, Mann M (2009) Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomics. Mol Cell Proteomics 8, 2796-808 19651622 Curated Info
34	Oppermann FS, et al. (2009) Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics 8, 1751-64 19369195 Curated Info
35	Tsai CF, et al. (2008) Immobilized metal affinity chromatography revisited: pH/acid control toward high selectivity in phosphoproteomics. J Proteome Res 7, 4058-69 18707149 Curated Info
36	Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48 18691976 Curated Info
37	Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7 18669648 Curated Info
38	Thingholm TE, et al. (2008) TiO2-Based Phosphoproteomic Analysis of the Plasma Membrane and the Effects of Phosphatase Inhibitor Treatment. J Proteome Res 7, 3304-3313 18578522 Curated Info
39	Ruse CI, et al. (2008) Motif-specific sampling of phosphoproteomes. J Proteome Res 7, 2140-50 18452278 Curated Info
40	Zahedi RP, et al. (2008) Phosphoproteome of resting human platelets. J Proteome Res 7, 526-34 18088087 Curated Info
41	Olsen JV, et al. (2006) Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell 127, 635-48 17081983 Curated Info