Ser575
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Home > Phosphorylation Site Page: > Ser575  -  RBM12B (human)

Site Information
PPEDFRHsPEDFRRP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 470958
Available spectra:  10 CST

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 20 , 21 , 22 , 23 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 )
Disease tissue studied:
breast cancer ( 5 , 23 ) , breast ductal carcinoma ( 5 ) , HER2 positive breast cancer ( 2 ) , luminal A breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, surrounding tissue ( 2 ) , breast cancer, triple negative ( 2 , 5 ) , cervical cancer ( 39 ) , cervical adenocarcinoma ( 39 ) , lung cancer ( 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 23 ) , non-small cell lung cancer ( 10 , 11 , 12 , 14 , 15 , 16 , 20 , 21 , 23 ) , non-small cell lung adenocarcinoma ( 10 , 11 , 12 , 13 , 14 , 15 , 16 , 21 ) , non-small cell large cell lung carcinoma ( 11 , 14 ) , non-small cell squamous cell lung carcinoma ( 13 ) , small-cell lung cancer ( 8 , 9 ) , lymphoma ( 6 ) , Burkitt's lymphoma ( 6 ) , follicular lymphoma ( 6 ) , mantle cell lymphoma ( 6 ) , ovarian cancer ( 5 ) , prostate cancer ( 38 ) , melanoma skin cancer ( 3 )
Relevant cell line - cell type - tissue:
293E (epithelial) ( 31 ) , A549 (pulmonary) ( 10 , 17 ) , BJAB (B lymphocyte) ( 6 ) , breast ( 2 , 5 ) , BT-549 (breast cell) ( 23 ) , Cal-12T (pulmonary) ( 16 ) , Calu 6 (pulmonary) ( 23 ) , Calu-3 (pulmonary) ( 12 ) , DMS153 (pulmonary) ( 15 ) , DMS53 (pulmonary) ( 9 ) , DMS79 (pulmonary) ( 8 ) , FL-18 (B lymphocyte) ( 6 ) , H2009 (pulmonary) ( 23 ) , H2077 (pulmonary) ( 23 ) , H2887 (pulmonary) ( 23 ) , H322M (pulmonary) ( 23 ) , HCC15 (pulmonary) ( 13 ) , HCC1937 (breast cell) ( 23 ) , HCC44 (pulmonary) ( 16 ) , HCC78 (pulmonary) ( 14 ) , HCC827 (pulmonary) ( 12 ) , HeLa (cervical) ( 1 , 4 , 22 , 35 , 36 , 41 , 42 , 43 ) , HeLa S3 (cervical) ( 39 ) , HUES-9 ('stem, embryonic') ( 34 ) , JEKO-1 (B lymphocyte) ( 6 ) , Jurkat (T lymphocyte) ( 18 , 25 , 26 , 27 , 28 , 29 , 30 , 32 , 33 , 37 ) , K562 (erythroid) ( 22 , 40 ) , liver ( 7 ) , LNCaP (prostate cell) ( 38 ) , LOU-NH91 (squamous) ( 13 ) , lung ( 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 ) , MCF-7 (breast cell) ( 23 ) , MDA-MB-231 (breast cell) ( 23 ) , NCEB-1 (B lymphocyte) ( 6 ) , NCI-H128 (pulmonary) ( 8 ) , NCI-H1299 (pulmonary) ( 11 ) , NCI-H1355 (pulmonary) ( 10 ) , NCI-H1417 (pulmonary) ( 8 ) , NCI-H1437 (pulmonary) ( 15 , 16 ) , NCI-H157 (pulmonary) ( 23 ) , NCI-H1648 (pulmonary) ( 23 ) , NCI-H1650 (pulmonary) ( 12 ) , NCI-H1666 (pulmonary) ( 16 , 23 ) , NCI-H1703 (squamous) ( 13 ) , NCI-H1734 (pulmonary) ( 11 ) , NCI-H1781 (pulmonary) ( 14 ) , NCI-H1792 (pulmonary) ( 10 ) , NCI-H1944 (pulmonary) ( 11 ) , NCI-H1975 (pulmonary) ( 12 ) , NCI-H2073 (pulmonary) ( 13 , 15 ) , NCI-H209 (pulmonary) ( 8 , 15 ) , NCI-H2106 (pulmonary) ( 12 ) , NCI-H2228 (pulmonary) ( 14 , 21 ) , NCI-H23 (pulmonary) ( 10 ) , NCI-H2342 (pulmonary) ( 13 ) , NCI-H2405 (pulmonary) ( 16 ) , NCI-H3122 (pulmonary) ( 14 , 20 ) , NCI-H358 (pulmonary) ( 11 ) , NCI-H441 (pulmonary) ( 10 ) , NCI-H446 (pulmonary) ( 9 ) , NCI-H460 (pulmonary) ( 11 ) , NCI-H520 (squamous) ( 23 ) , NCI-H524 (pulmonary) ( 8 ) , NCI-H526 (pulmonary) ( 9 ) , NCI-H661 (pulmonary) ( 14 ) , NCI-H69 (pulmonary) ( 9 ) , NCI-H82 (pulmonary) ( 9 ) , NCI-H838 (pulmonary) ( 15 ) , OCI-ly1 (B lymphocyte) ( 6 ) , ovary ( 5 ) , PC9 (pulmonary) ( 23 ) , Raji (B lymphocyte) ( 6 ) , SU-DHL-4 (B lymphocyte) ( 6 ) , WM239A (melanocyte) ( 3 )

References 

1

Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296
27281782   Curated Info

2

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

3

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

4

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

5

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

6

Rolland D, et al. (2014) Global phosphoproteomic profiling reveals distinct signatures in B-cell non-Hodgkin lymphomas. Am J Pathol 184, 1331-42
24667141   Curated Info

7

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

8

Rikova K, Hall B (2013) CST Curation Set: 20734, 21161, 30112, 30153, 30154; Year: 2013; Biosample/Treatment: cell line, H1417, DMS79, H128, H209, H524; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

9

Rikova K, Hall B (2013) CST Curation Set: 20735, 21162, 30155, 30156, 30157; Year: 2013; Biosample/Treatment: cell line, DMS53, H526, H69, H82, H446; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

10

Rikova K, Hall B (2013) CST Curation Set: 20736, 21163, 30158, 30159, 30160; Year: 2013; Biosample/Treatment: cell line, A549, H1355, H23, H441, H1792; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

11

Rikova K, Hall B (2013) CST Curation Set: 20737, 21164, 30161, 30162, 30163; Year: 2013; Biosample/Treatment: cell line, H1299, H1944, H358, H1734, H460; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

12

Rikova K, Hall B (2013) CST Curation Set: 20738, 21165, 30164, 30165, 30166; Year: 2013; Biosample/Treatment: cell line, H1650, HCC827, H1975, Calu-3, H2106; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

13

Rikova K, Hall B (2013) CST Curation Set: 20739, 21166, 30167, 30168, 30169; Year: 2013; Biosample/Treatment: cell line, H2342, H2073, Lou-NH91, HCC15, H1703; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

14

Rikova K, Hall B (2013) CST Curation Set: 20740, 21167, 30170, 30171, 30172; Year: 2013; Biosample/Treatment: cell line, H2228, H3122, HCC78, H661, H1781; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

15

Rikova K, Hall B (2013) CST Curation Set: 20742, 21169, 30176, 30177, 30178; Year: 2013; Biosample/Treatment: cell line, H838, DMS153, H2073, H209, H1437; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

16

Rikova K, Hall B (2013) CST Curation Set: 20741, 21168, 30173, 30174, 30175; Year: 2013; Biosample/Treatment: cell line, H1666, CAL-12T, H2405, HCC44, H1437; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

17

Kim JY, et al. (2013) Dissection of TBK1 signaling via phosphoproteomics in lung cancer cells. Proc Natl Acad Sci U S A 110, 12414-9
23836654   Curated Info

18

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

19

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

20

Rikova K (2013) CST Curation Set: 18860; Year: 2012; Biosample/Treatment: cell line, H3122/crizotinib, geldanamycin; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pT
Curated Info

21

Rikova K (2013) CST Curation Set: 18859; Year: 2013; Biosample/Treatment: cell line, H2228/crizotinib, geldanamycin; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pT
Curated Info

22

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

23

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

24

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

25

Mulhern D (2012) CST Curation Set: 13339; Year: 2012; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

26

Mulhern D (2011) CST Curation Set: 12706; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: DpSG
Curated Info

27

Mulhern D (2011) CST Curation Set: 12641; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

28

Mulhern D (2011) CST Curation Set: 12642; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

29

Mulhern D (2011) CST Curation Set: 12577; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: DpSG
Curated Info

30

Mulhern D (2011) CST Curation Set: 12439; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

31

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

32

Guo A (2011) CST Curation Set: 12065; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

33

Guo A (2011) CST Curation Set: 12066; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

34

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

35

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

36

Zhou J (2010) CST Curation Set: 10708; Year: 2010; Biosample/Treatment: cell line, HeLa/untreated; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: PXpSP, pSPX(K/R) Antibodies Used to Purify Peptides prior to LCMS: Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, PTMScan(R) Phospho-MAPK/CDK Substrate Motif (PXS*P, S*PXK/R) Immunoaffinity Beads Cat#: 1982
Curated Info

37

Possemato A (2010) CST Curation Set: 9285; Year: 2010; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

38

Chen L, Giorgianni F, Beranova-Giorgianni S (2010) Characterization of the phosphoproteome in LNCaP prostate cancer cells by in-gel isoelectric focusing and tandem mass spectrometry. J Proteome Res 9, 174-8
20044836   Curated Info

39

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

40

Pan C, Olsen JV, Daub H, Mann M (2009) Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomics. Mol Cell Proteomics 8, 2796-808
19651622   Curated Info

41

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
18669648   Curated Info

42

Ruse CI, et al. (2008) Motif-specific sampling of phosphoproteomes. J Proteome Res 7, 2140-50
18452278   Curated Info

43

Olsen JV, et al. (2006) Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell 127, 635-48
17081983   Curated Info