Ser228

Site Information
EDEEVEEsDLsDFED SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 470743

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 )
Disease tissue studied:	melanoma skin cancer ( 7 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 2 ) , 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 6 ) , 32Dcl3 (myeloid) ( 6 ) , 3T3 (fibroblast) [CDC42 (human), transfection] ( 1 ) , 3T3 (fibroblast) [KRas (human), transfection] ( 1 ) , 3T3 (fibroblast) ( 1 ) , heart ( 3 ) , Hepa 1-6 (epithelial) ( 8 ) , macrophage-bone marrow ( 5 ) , macrophage-bone marrow [DUSP1 (mouse), homozygous knockout] ( 5 ) , MEF (fibroblast) [Raptor (mouse), knockdown] ( 4 ) , MEF (fibroblast) [RICTOR (mouse), knockdown] ( 4 ) , MEF (fibroblast) ( 4 ) , skin [mGluR1 (mouse), transgenic, TG mutant mice] ( 7 )

References
1	Gnad F, et al. (2013) Systems-wide Analysis of K-Ras, Cdc42, and PAK4 Signaling by Quantitative Phosphoproteomics. Mol Cell Proteomics 12, 2070-80 23608596 Curated Info
2	Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20 23684622 Curated Info
3	Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11 23737553 Curated Info
4	Robitaille AM, et al. (2013) Quantitative phosphoproteomics reveal mTORC1 activates de novo pyrimidine synthesis. Science 339, 1320-3 23429704 Curated Info
5	Weintz G, et al. (2010) The phosphoproteome of toll-like receptor-activated macrophages. Mol Syst Biol 6, 371 20531401 Curated Info
6	Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39 19854140 Curated Info
7	Zanivan S, et al. (2008) Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry. J Proteome Res 7, 5314-26 19367708 Curated Info
8	Pan C, Gnad F, Olsen JV, Mann M (2008) Quantitative phosphoproteome analysis of a mouse liver cell line reveals specificity of phosphatase inhibitors. Proteomics 8, 4534-46 18846507 Curated Info