Ser662
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Home > Phosphorylation Site Page: > Ser662  -  PKCD (rat)

Site Information
QtAFKGFsFVNPKYE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447580

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 6 , 10 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 7 , 8 , 9 ) , mutation of modification site ( 9 ) , phospho-antibody ( 1 , 6 , 9 , 10 ) , western blotting ( 6 , 9 , 10 )
Relevant cell line - cell type - tissue:
'brain, cerebellum' ( 3 ) , 'brain, cerebral cortex' ( 3 ) , 'kidney, cortex' ( 4 ) , adipose tissue ( 3 ) , BCEC4 (endothelial) ( 7 ) , COS (fibroblast) ( 9 ) , heart ( 3 ) , hepatocyte ( 6 ) , IEC-6 (epithelial) ( 2 ) , intestine ( 3 ) , kidney ( 3 ) , liver ( 3 , 5 , 8 ) , lung ( 3 ) , myocyte-heart ( 1 ) , pancreas ( 3 ) , parotid acinar-salivary gland ( 10 ) , spleen ( 3 ) , stomach ( 3 ) , testis ( 3 ) , thymus ( 3 )

Upstream Regulation
Putative in vivo kinases:
PKCD (rat) ( 6 )
Phosphatases, in vitro:
PTP1B (human) ( 10 )
Treatments:
carbachol ( 10 ) , H2O2 ( 9 ) , LY294002 ( 10 ) , phorbol_ester ( 6 , 10 ) , racepinefrine ( 6 ) , rapamycin ( 10 ) , wortmannin ( 10 )

References 

1

Gong J, Holewinski RJ, Van Eyk JE, Steinberg SF (2016) A novel phosphorylation site at Ser130 adjacent to the pseudosubstrate domain contributes to the activation of protein kinase C-δ. Biochem J 473, 311-20
26546672   Curated Info

2

Courcelles M, et al. (2013) Phosphoproteome dynamics reveal novel ERK1/2 MAP kinase substrates with broad spectrum of functions. Mol Syst Biol 9, 669
23712012   Curated Info

3

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

4

Feric M, et al. (2011) Large-scale phosphoproteomic analysis of membrane proteins in renal proximal and distal tubule. Am J Physiol Cell Physiol 300, C755-70
21209370   Curated Info

5

Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729
21738781   Curated Info

6

Robles-Flores M, et al. (2008) Posttranslational modifications on protein kinase c isozymes. Effects of epinephrine and phorbol esters. Biochim Biophys Acta 1783, 695-712
18295358   Curated Info

7

Klemm C, et al. (2006) Evaluation of the titanium dioxide approach for MS analysis of phosphopeptides. J Mass Spectrom 41, 1623-32
17089331   Curated Info

8

Moser K, White FM (2006) Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS. J Proteome Res 5, 98-104
16396499   Curated Info

9

Konishi H, et al. (2001) Phosphorylation sites of protein kinase C delta in H2O2-treated cells and its activation by tyrosine kinase in vitro. Proc Natl Acad Sci U S A 98, 6587-92
11381116   Curated Info

10

Benes C, Soltoff SP (2001) Modulation of PKCdelta tyrosine phosphorylation and activity in salivary and PC-12 cells by Src kinases. Am J Physiol Cell Physiol 280, C1498-510
11350745   Curated Info