Lys63

Site Information
AMBIGUOUS SITES: RPS27A (human): , UBB (human): , UBB (human): , UBB (human): , UBC (human): , UBC (human): , UBC (human): , UBC (human): , UBC (human): , UBC (human): , UBC (human): , UBC (human): , UBC (human): , ubiquitin (human):
LsDyNIQkEstLHLV SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 55461700

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 2 , 3 )
Relevant cell line - cell type - tissue:	293N3S (epithelial) ( 3 ) , HeLa (cervical) [SUMO2 (human), transfection, SUMO2 K0 Q87R (lysine-deficient SUMO2, with Q87R to create convenient Trypsin cleavage site)] ( 2 )

References
1	Lumpkin RJ, et al. (2017) Site-specific identification and quantitation of endogenous SUMO modifications under native conditions. Nat Commun 8, 1171 29079793 Curated Info
2	Hendriks IA, et al. (2014) Uncovering global SUMOylation signaling networks in a site-specific manner. Nat Struct Mol Biol 21, 927-36 25218447 Curated Info
3	Tammsalu T, et al. (2014) Proteome-wide identification of SUMO2 modification sites. Sci Signal 7, rs2 24782567 Curated Info