Lys125

Site Information
ADFQNNEkFVQFkQQ SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 55435117

In vivo Characterization
Methods used to characterize site in vivo:	immunoprecipitation ( 1 ) , mass spectrometry ( 1 , 2 , 3 ) , mutation of modification site ( 1 ) , western blotting ( 1 )
Relevant cell line - cell type - tissue:	293 (epithelial) ( 1 ) , 293N3S (epithelial) ( 3 ) , HeLa (cervical) [SUMO2 (human), transfection, SUMO2 K0 Q87R (lysine-deficient SUMO2, with Q87R to create convenient Trypsin cleavage site)] ( 2 ) , HeLa (cervical) ( 1 )

References
1	Li C, et al. (2020) Quantitative SUMO proteomics identifies PIAS1 substrates involved in cell migration and motility. Nat Commun 11, 834 32047143 Curated Info
2	Hendriks IA, et al. (2014) Uncovering global SUMOylation signaling networks in a site-specific manner. Nat Struct Mol Biol 21, 927-36 25218447 Curated Info
3	Tammsalu T, et al. (2014) Proteome-wide identification of SUMO2 modification sites. Sci Signal 7, rs2 24782567 Curated Info