Ser312
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Home > Phosphorylation Site Page: > Ser312  -  ADRB1 (human)

Site Information
RAGKRRPsRLVALRE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 469625

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 5 , 6 , 7 ) , immunoprecipitation ( 1 , 2 , 3 , 5 ) , mass spectrometry ( 2 ) , mutation of modification site ( 1 , 2 , 3 , 4 , 5 , 6 , 7 ) , phospho-antibody ( 1 ) , western blotting ( 1 , 3 )
Relevant cell line - cell type - tissue:
293 (epithelial) [ADRB1 (human)] ( 7 ) , 293 (epithelial) ( 1 , 2 , 3 , 5 , 6 ) , myocyte-heart [AKAP5 (mouse), homozygous knockout] ( 4 ) , SK-N-MC (neural crest) ( 6 )

Upstream Regulation
Putative in vivo kinases:
PKACA (human) ( 3 , 5 , 6 )
Treatments:
H-89 ( 3 ) , isoproterenol ( 5 , 6 , 7 ) , mPKI ( 3 ) , siRNA ( 5 )

Downstream Regulation
Effects of modification on ADRB1:
intracellular localization ( 1 ) , molecular association, regulation ( 5 ) , receptor desensitization, altered ( 5 , 6 ) , receptor internalization, induced ( 3 ) , receptor recycling, altered ( 5 , 7 ) , receptor recycling, induced ( 3 , 4 )
Induce interaction with:
DLG1 (human) ( 5 )

References 

1

Nooh MM, Bahouth SW (2017) Two barcodes encoded by the type-1 PDZ and by phospho-Ser(312) regulate retromer/WASH-mediated sorting of the ß1-adrenergic receptor from endosomes to the plasma membrane. Cell Signal 29, 192-208
27816670   Curated Info

2

Hinz L, et al. (2017) Two serines in the distal C-terminus of the human ß1-adrenoceptor determine ß-arrestin2 recruitment. PLoS One 12, e0176450
28472170   Curated Info

3

Nooh MM, Chumpia MM, Hamilton TB, Bahouth SW (2014) Sorting of β1-Adrenergic Receptors Is Mediated by Pathways That Are Either Dependent on or Independent of Type I PDZ, Protein Kinase A (PKA), and SAP97. J Biol Chem 289, 2277-94
24324269   Curated Info

4

Li X, Nooh MM, Bahouth SW (2013) Role of AKAP79/150 Protein in β1-Adrenergic Receptor Trafficking and Signaling in Mammalian Cells. J Biol Chem 288, 33797-812
24121510   Curated Info

5

Gardner LA, Naren AP, Bahouth SW (2007) Assembly of an SAP97-AKAP79-cAMP-dependent protein kinase scaffold at the type 1 PSD-95/DLG/ZO1 motif of the human beta(1)-adrenergic receptor generates a receptosome involved in receptor recycling and networking. J Biol Chem 282, 5085-99
17170109   Curated Info

6

Gardner LA, et al. (2006) AKAP79-mediated targeting of the cyclic AMP-dependent protein kinase to the beta1-adrenergic receptor promotes recycling and functional resensitization of the receptor. J Biol Chem 281, 33537-53
16940053   Curated Info

7

Gardner LA, et al. (2004) Role of the cyclic AMP-dependent protein kinase in homologous resensitization of the beta1-adrenergic receptor. J Biol Chem 279, 21135-43
14990580   Curated Info