Ser40
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Home > Phosphorylation Site Page: > Ser40  -  TH (mouse)

Site Information
RFIGRRQsLIEDARK   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448545

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 10 ) , mass spectrometry ( 5 , 6 , 8 , 9 ) , mutation of modification site ( 4 ) , phospho-antibody ( 2 , 4 , 7 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 20 , 21 , 22 ) , western blotting ( 2 , 4 , 7 , 10 , 11 , 13 , 14 , 15 , 16 , 20 , 22 )
Disease tissue studied:
neuroblastoma ( 6 , 7 )
Relevant cell line - cell type - tissue:
'brain, cerebellum' ( 21 ) , 'brain, nucleus accumbens' ( 16 ) , 'brain, striatum' ( 2 , 11 , 13 , 15 , 18 , 20 , 22 ) , 'brain, ventral tegmental area' ( 16 ) , 'fat, brown' ( 8 ) , 'neuron, cortical'-brain ( 17 ) , 'neuron, substantia nigra'-brain ( 14 ) , brain ( 8 , 9 ) , heart ( 8 , 12 ) , kidney ( 8 ) , liver ( 5 ) , MN9D ('neuron, mesencephalic') ( 10 ) , N1E-115 (neuron) ( 6 ) , N27 (neuron) ( 14 ) , Neuro-2a (neuron) ( 7 ) , PC-12 (chromaffin) ( 14 )

Upstream Regulation
Regulatory protein:
IREB2 (mouse) ( 18 ) , PPP2R5A (mouse) ( 7 ) , SNCA (human) ( 4 , 10 ) , SNCA (mouse) ( 17 )
Kinases, in vitro:
PKACA (human) ( 19 )
Putative upstream phosphatases:
PPP2CA (mouse) ( 14 )
Treatments:
caffeine ( 15 ) , CGS_21680 ( 15 ) , clozapine ( 20 ) , colforsin ( 15 ) , eticlopride ( 20 ) , haloperidol ( 11 , 20 ) , isoflurane ( 13 ) , ketamine ( 13 ) , leptin ( 16 ) , okadaic_acid ( 14 ) , papaverine ( 11 ) , phorbol_ester ( 7 ) , propofol ( 13 ) , quinpirole ( 22 ) , raclopride ( 17 ) , rolipram ( 11 ) , rottlerin ( 7 , 14 ) , SL327 ( 20 ) , tyrosine ( 17 )

Downstream Regulation
Effects of modification on TH:
enzymatic activity, induced ( 12 , 14 )
Effects of modification on biological processes:
neural plasticity ( 2 )

Disease / Diagnostics Relevance
Relevant diseases:
obesity, hyperphagic ( 16 )

References 

1

Wasinski F, et al. (2021) Growth hormone receptor in dopaminergic neurones regulates stress-induced prolactin release in male mice. J Neuroendocrinol 33, e12957
33769619   Curated Info

2

Becanovic K, et al. (2020) Effects of a novel psychomotor stabilizer, IRL790, on biochemical measures of synaptic markers and neurotransmission. J Pharmacol Exp Ther
32358047   Curated Info

3

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

4

Hua G, et al. (2015) Protein Phosphatase 2A is Involved in the Tyrosine Hydroxylase Phosphorylation Regulated by α-Synuclein. Neurochem Res 40, 428-37
25567480   Curated Info

5

Wilson-Grady JT, Haas W, Gygi SP (2013) Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation. Methods 61, 277-86
23567750   Curated Info

6

Wang Y, et al. (2011) Spatial phosphoprotein profiling reveals a compartmentalized extracellular signal-regulated kinase switch governing neurite growth and retraction. J Biol Chem 286, 18190-201
21454597   Curated Info

7

Ahn JH, et al. (2011) Protein kinase C-dependent dephosphorylation of tyrosine hydroxylase requires the B56δ heterotrimeric form of protein phosphatase 2A. PLoS One 6, e26292
22046270   Curated Info

8

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

9

Wiśniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9
20415495   Curated Info

10

Lou H, et al. (2010) Serine 129 phosphorylation reduces the ability of alpha-synuclein to regulate tyrosine hydroxylase and protein phosphatase 2A in vitro and in vivo. J Biol Chem 285, 17648-61
20356833   Curated Info

11

Nishi A, et al. (2008) Distinct roles of PDE4 and PDE10A in the regulation of cAMP/PKA signaling in the striatum. J Neurosci 28, 10460-71
18923023   Curated Info

12

Hoard JL, et al. (2008) Cholinergic neurons of mouse intrinsic cardiac ganglia contain noradrenergic enzymes, norepinephrine transporters, and the neurotrophin receptors tropomyosin-related kinase A and p75. Neuroscience 156, 129-42
18674600   Curated Info

13

Snyder GL, Galdi S, Hendrick JP, Hemmings HC (2007) General anesthetics selectively modulate glutamatergic and dopaminergic signaling via site-specific phosphorylation in vivo. Neuropharmacology 53, 619-30
17826804   Curated Info

14

Zhang D, et al. (2007) Protein kinase C delta negatively regulates tyrosine hydroxylase activity and dopamine synthesis by enhancing protein phosphatase-2A activity in dopaminergic neurons. J Neurosci 27, 5349-62
17507557   Curated Info

15

Sahin B, et al. (2007) Evaluation of neuronal phosphoproteins as effectors of caffeine and mediators of striatal adenosine A2A receptor signaling. Brain Res 1129, 1-14
17157277   Curated Info

16

Fulton S, et al. (2006) Leptin regulation of the mesoaccumbens dopamine pathway. Neuron 51, 811-22
16982425   Curated Info

17

Drolet RE, Behrouz B, Lookingland KJ, Goudreau JL (2006) Substrate-mediated enhancement of phosphorylated tyrosine hydroxylase in nigrostriatal dopamine neurons: evidence for a role of alpha-synuclein. J Neurochem 96, 950-9
16412098   Curated Info

18

Salvatore MF, et al. (2005) Neurochemical investigations of dopamine neuronal systems in iron-regulatory protein 2 (IRP-2) knockout mice. Brain Res Mol Brain Res 139, 341-7
16051392   Curated Info

19

Kansy JW, et al. (2004) Identification of tyrosine hydroxylase as a physiological substrate for Cdk5. J Neurochem 91, 374-84
15447670   Curated Info

20

Håkansson K, et al. (2004) Regulation of striatal tyrosine hydroxylase phosphorylation by acute and chronic haloperidol. Eur J Neurosci 20, 1108-12
15305880   Curated Info

21

Sawada K, et al. (2004) Abnormal expression of tyrosine hydroxylase not accompanied by phosphorylation at serine 40 in cerebellar Purkinje cells of ataxic mutant mice, rolling mouse Nagoya and dilute-lethal. Congenit Anom (Kyoto) 44, 46-50
15008900   Curated Info

22

Lindgren N, et al. (2003) Distinct roles of dopamine D2L and D2S receptor isoforms in the regulation of protein phosphorylation at presynaptic and postsynaptic sites. Proc Natl Acad Sci U S A 100, 4305-9
12651945   Curated Info