Ser31
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Home > Phosphorylation Site Page: > Ser31  -  TH (mouse)

Site Information
KQAEAVtsPRFIGRR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448544

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 2 , 3 , 5 , 6 , 7 ) , mutation of modification site ( 12 ) , peptide sequencing ( 11 ) , phospho-antibody ( 1 , 4 , 8 , 9 , 10 , 13 , 14 ) , western blotting ( 1 , 4 , 8 , 11 , 13 , 14 )
Disease tissue studied:
neuroblastoma ( 3 , 4 )
Relevant cell line - cell type - tissue:
'brain, cerebral cortex' ( 7 ) , 'brain, striatum' ( 1 , 10 , 13 , 14 ) , 'brain, ventral tegmental area' ( 11 ) , 'fat, brown' ( 5 ) , 'neuron, cortical'-brain ( 9 ) , 'neuron, substantia nigra'-brain ( 8 ) , brain ( 2 , 6 ) , N1E-115 (neuron) ( 3 ) , N27 (neuron) ( 8 ) , Neuro-2a (neuron) ( 4 ) , PC-12 (chromaffin) ( 8 )

Upstream Regulation
Regulatory protein:
CLOCK (mouse) ( 11 ) , IREB2 (mouse) ( 10 ) , PPP2R5A (mouse) ( 4 ) , SNCA (mouse) ( 9 )
Putative in vivo kinases:
CDK5 (mouse) ( 12 , 13 ) , ERK1 (mouse) ( 13 ) , ERK2 (mouse) ( 13 )
Kinases, in vitro:
CDK5 (human) ( 13 ) , ERK1 (human) ( 13 ) , ERK2 (human) ( 13 )
Treatments:
clozapine ( 14 ) , depolarization ( 13 ) , eticlopride ( 14 ) , haloperidol ( 14 ) , phorbol_ester ( 4 ) , raclopride ( 9 ) , seliciclib ( 13 ) , SL327 ( 14 ) , tyrosine ( 9 ) , U0126 ( 13 )

Downstream Regulation
Effects of modification on TH:
enzymatic activity, induced ( 12 ) , protein stabilization ( 12 )
Effects of modification on biological processes:
neural plasticity ( 1 )

References 

1

Becanovic K, et al. (2020) Effects of a novel psychomotor stabilizer, IRL790, on biochemical measures of synaptic markers and neurotransmission. J Pharmacol Exp Ther
32358047   Curated Info

2

Trinidad JC, et al. (2012) Global identification and characterization of both O-GlcNAcylation and phosphorylation at the murine synapse. Mol Cell Proteomics 11, 215-29
22645316   Curated Info

3

Wang Y, et al. (2011) Spatial phosphoprotein profiling reveals a compartmentalized extracellular signal-regulated kinase switch governing neurite growth and retraction. J Biol Chem 286, 18190-201
21454597   Curated Info

4

Ahn JH, et al. (2011) Protein kinase C-dependent dephosphorylation of tyrosine hydroxylase requires the B56δ heterotrimeric form of protein phosphatase 2A. PLoS One 6, e26292
22046270   Curated Info

5

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

6

Wiśniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9
20415495   Curated Info

7

Tweedie-Cullen RY, Reck JM, Mansuy IM (2009) Comprehensive mapping of post-translational modifications on synaptic, nuclear, and histone proteins in the adult mouse brain. J Proteome Res 8, 4966-82
19737024   Curated Info

8

Zhang D, et al. (2007) Protein kinase C delta negatively regulates tyrosine hydroxylase activity and dopamine synthesis by enhancing protein phosphatase-2A activity in dopaminergic neurons. J Neurosci 27, 5349-62
17507557   Curated Info

9

Drolet RE, Behrouz B, Lookingland KJ, Goudreau JL (2006) Substrate-mediated enhancement of phosphorylated tyrosine hydroxylase in nigrostriatal dopamine neurons: evidence for a role of alpha-synuclein. J Neurochem 96, 950-9
16412098   Curated Info

10

Salvatore MF, et al. (2005) Neurochemical investigations of dopamine neuronal systems in iron-regulatory protein 2 (IRP-2) knockout mice. Brain Res Mol Brain Res 139, 341-7
16051392   Curated Info

11

McClung CA, et al. (2005) Regulation of dopaminergic transmission and cocaine reward by the Clock gene. Proc Natl Acad Sci U S A 102, 9377-81
15967985   Curated Info

12

Moy LY, Tsai LH (2004) Cyclin-dependent kinase 5 phosphorylates serine 31 of tyrosine hydroxylase and regulates its stability. J Biol Chem 279, 54487-93
15471880   Curated Info

13

Kansy JW, et al. (2004) Identification of tyrosine hydroxylase as a physiological substrate for Cdk5. J Neurochem 91, 374-84
15447670   Curated Info

14

Håkansson K, et al. (2004) Regulation of striatal tyrosine hydroxylase phosphorylation by acute and chronic haloperidol. Eur J Neurosci 20, 1108-12
15305880   Curated Info