Ser632
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser632  -  eNOS (mouse)

Site Information
WRRKRKEsSNtDSAG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448637

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 3 , 5 ) , phospho-antibody ( 4 , 6 ) , western blotting ( 4 , 6 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 1 , 3 ) , MEF (fibroblast) ( 4 ) , thoracic aorta ( 6 )

Upstream Regulation
Regulatory protein:
AMPKA2 (mouse) ( 4 )
Treatments:
angiotensin_2 ( 6 ) , blood pressure ( 6 ) , H-89 ( 6 ) , icatibant ( 6 ) , insulin ( 3 ) , KT5720 ( 6 ) , LY294002 ( 6 ) , PD123319 ( 6 ) , wortmannin ( 6 )

References 

1

Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6
26060331   Curated Info

2

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

3

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

4

Chen Z, et al. (2010) Shear stress, SIRT1, and vascular homeostasis. Proc Natl Acad Sci U S A 107, 10268-73
20479254   Curated Info

5

Rikova K (2008) CST Curation Set: 4512; Year: 2008; Biosample/Treatment: cell line, MD Ander a0859/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

6

Yayama K, Hiyoshi H, Imazu D, Okamoto H (2006) Angiotensin II stimulates endothelial NO synthase phosphorylation in thoracic aorta of mice with abdominal aortic banding via type 2 receptor. Hypertension 48, 958-64
17000928   Curated Info