Ser633
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Home > Phosphorylation Site Page: > Ser633  -  eNOS (human)

Site Information
WRRKRKEssNtDsAG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448637
Available spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 , 2 ) , mass spectrometry ( 1 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 15 , 17 ) , mutation of modification site ( 1 , 17 ) , phospho-antibody ( 1 , 2 , 14 , 16 , 17 , 19 ) , phosphopeptide mapping ( 17 ) , western blotting ( 1 , 2 , 14 , 17 , 19 )
Disease tissue studied:
breast cancer ( 3 ) , breast cancer, triple negative ( 3 ) , hepatocellular carcinoma, surrounding tissue ( 15 ) , lung cancer ( 5 , 6 , 7 , 8 , 9 , 10 , 11 ) , non-small cell lung cancer ( 7 , 9 , 10 ) , non-small cell lung adenocarcinoma ( 7 , 8 , 9 , 10 , 11 ) , non-small cell large cell lung carcinoma ( 9 ) , non-small cell squamous cell lung carcinoma ( 8 ) , small-cell lung cancer ( 5 , 6 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
Cortactin (human) ( 1 ) , PTEN (human) ( 19 )
Putative in vivo kinases:
AMPKA1 (human) ( 14 ) , Pim1 (human) ( 2 )
Kinases, in vitro:
Pim1 (human) ( 2 ) , PKACA (human) ( 17 , 18 ) , PKG2 (human) ( 18 )
Treatments:
compound_C ( 14 ) , H-89 ( 2 ) , high_glucose ( 2 ) , histamine ( 2 ) , nicotinamide ( 14 ) , pulsatile shear stress ( 1 ) , rosuvastatin ( 12 ) , siRNA ( 2 ) , SMI-4a ( 2 ) , thrombin ( 2 ) , VEGF ( 2 ) , wortmannin ( 2 )

Downstream Regulation
Effects of modification on eNOS:
enzymatic activity, induced ( 2 )

References 

1

Shentu TP, et al. (2016) AMP-Activated Protein Kinase and Sirtuin 1 Coregulation of Cortactin Contributes to Endothelial Function. Arterioscler Thromb Vasc Biol 36, 2358-2368
27758765   Curated Info

2

Chen M, et al. (2016) Pim1 kinase promotes angiogenesis through phosphorylation of endothelial nitric oxide synthase at Ser-633. Cardiovasc Res 109, 141-50
26598507   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

5

Rikova K, Hall B (2013) CST Curation Set: 20734, 21161, 30112, 30153, 30154; Year: 2013; Biosample/Treatment: cell line, H1417, DMS79, H128, H209, H524; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

6

Rikova K, Hall B (2013) CST Curation Set: 20735, 21162, 30155, 30156, 30157; Year: 2013; Biosample/Treatment: cell line, DMS53, H526, H69, H82, H446; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

7

Rikova K, Hall B (2013) CST Curation Set: 20736, 21163, 30158, 30159, 30160; Year: 2013; Biosample/Treatment: cell line, A549, H1355, H23, H441, H1792; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

8

Rikova K, Hall B (2013) CST Curation Set: 20739, 21166, 30167, 30168, 30169; Year: 2013; Biosample/Treatment: cell line, H2342, H2073, Lou-NH91, HCC15, H1703; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

9

Rikova K, Hall B (2013) CST Curation Set: 20740, 21167, 30170, 30171, 30172; Year: 2013; Biosample/Treatment: cell line, H2228, H3122, HCC78, H661, H1781; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

10

Rikova K, Hall B (2013) CST Curation Set: 20741, 21168, 30173, 30174, 30175; Year: 2013; Biosample/Treatment: cell line, H1666, CAL-12T, H2405, HCC44, H1437; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

11

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
23911959   Curated Info

12

Huang B, et al. (2013) Rosuvastatin-regulated post-translational phosphoproteome in human umbilical vein endothelial cells. Kaohsiung J Med Sci 29, 347-52
23768697   Curated Info

13

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

14

Chen Z, et al. (2010) Shear stress, SIRT1, and vascular homeostasis. Proc Natl Acad Sci U S A 107, 10268-73
20479254   Curated Info

15

Han G, et al. (2010) Phosphoproteome analysis of human liver tissue by long-gradient nanoflow LC coupled with multiple stage MS analysis. Electrophoresis 31, 1080-9
20166139   Curated Info

16

Fulton D, et al. (2004) Targeting of endothelial nitric-oxide synthase to the cytoplasmic face of the Golgi complex or plasma membrane regulates Akt- versus calcium-dependent mechanisms for nitric oxide release. J Biol Chem 279, 30349-57
15136572   Curated Info

17

Michell BJ, et al. (2002) Identification of regulatory sites of phosphorylation of the bovine endothelial nitric-oxide synthase at serine 617 and serine 635. J Biol Chem 277, 42344-51
12171920   Curated Info

18

Butt E, et al. (2000) Endothelial nitric-oxide synthase (type III) is activated and becomes calcium independent upon phosphorylation by cyclic nucleotide-dependent protein kinases. J Biol Chem 275, 5179-87
10671564   Curated Info

19

Church JE, et al. Inhibition of endothelial nitric oxide synthase by the lipid phosphatase PTEN. Vascul Pharmacol 52, 191-8
19962452   Curated Info