Thr147

Site Information
KKATGAAtPKKtAKK SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 5007339

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 3 , 4 , 5 , 6 , 7 , 8 )
Relevant cell line - cell type - tissue:	'brain, cerebral cortex' ( 6 ) , 3T3 (fibroblast) ( 8 ) , brain ( 5 ) , Hepa 1-6 (epithelial) ( 7 ) , macrophage-peritoneum ( 3 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 4 )

Upstream Regulation
Regulatory protein:	RIPK3 (mouse) ( 3 )

References
1	Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250 27841257 Curated Info
2	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
3	Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51 22942356 Curated Info
4	Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22 21659604 Curated Info
5	Wiśniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9 20415495 Curated Info
6	Tweedie-Cullen RY, Reck JM, Mansuy IM (2009) Comprehensive mapping of post-translational modifications on synaptic, nuclear, and histone proteins in the adult mouse brain. J Proteome Res 8, 4966-82 19737024 Curated Info
7	Pan C, Gnad F, Olsen JV, Mann M (2008) Quantitative phosphoproteome analysis of a mouse liver cell line reveals specificity of phosphatase inhibitors. Proteomics 8, 4534-46 18846507 Curated Info
8	Wang Z, Gucek M, Hart GW (2008) Cross-talk between GlcNAcylation and phosphorylation: site-specific phosphorylation dynamics in response to globally elevated O-GlcNAc. Proc Natl Acad Sci U S A 105, 13793-8 18779572 Curated Info