Ser2251
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Home > Phosphorylation Site Page: > Ser2251  -  RANBP2 (human)

Site Information
HAsPLAssPVRkNLF   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 468569

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ) , mass spectrometry (in vitro) ( 1 , 13 ) , mutation of modification site ( 1 ) , western blotting ( 1 )
Disease tissue studied:
lung cancer ( 3 , 5 , 7 ) , non-small cell lung adenocarcinoma ( 3 , 5 ) , melanoma skin cancer ( 2 )
Relevant cell line - cell type - tissue:
CL1-0 (pulmonary) ( 7 ) , CL1-1 (pulmonary) ( 7 ) , CL1-2 (pulmonary) ( 7 ) , CL1-5 (pulmonary) ( 7 ) , DG75 (B lymphocyte) ( 8 ) , HeLa (cervical) ( 1 , 9 , 11 , 12 ) , Jurkat (T lymphocyte) ( 10 ) , K562 (erythroid) ( 6 ) , liver ( 4 ) , lung ( 5 ) , PC9 (pulmonary) ( 3 ) , WM239A (melanocyte) ( 2 )

Upstream Regulation
Kinases, in vitro:
CDK1 (human) ( 1 , 13 )
Treatments:
selumetinib ( 2 ) , vemurafenib ( 2 )

Downstream Regulation
Effects of modification on RANBP2:
intracellular localization ( 1 ) , molecular association, regulation ( 1 )
Induce interaction with:
BICD2 (human) ( 1 )

References 

1

Baffet AD, Hu DJ, Vallee RB (2015) Cdk1 Activates Pre-mitotic Nuclear Envelope Dynein Recruitment and Apical Nuclear Migration in Neural Stem Cells. Dev Cell 33, 703-16
26051540   Curated Info

2

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

3

Tsai CF, et al. (2015) Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics. Nat Commun 6, 6622
25814448   Curated Info

4

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

5

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
23911959   Curated Info

6

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

7

Wang YT, et al. (2010) An informatics-assisted label-free quantitation strategy that depicts phosphoproteomic profiles in lung cancer cell invasion. J Proteome Res 9, 5582-97
20815410   Curated Info

8

Iliuk AB, et al. (2010) In-depth analyses of kinase-dependent tyrosine phosphoproteomes based on metal ion-functionalized soluble nanopolymers. Mol Cell Proteomics 9, 2162-72
20562096   Curated Info

9

Chen RQ, et al. (2009) CDC25B mediates rapamycin-induced oncogenic responses in cancer cells. Cancer Res 69, 2663-8
19276368   Curated Info

10

Mayya V, et al. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal 2, ra46
19690332   Curated Info

11

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
18669648   Curated Info

12

Ruse CI, et al. (2008) Motif-specific sampling of phosphoproteomes. J Proteome Res 7, 2140-50
18452278   Curated Info

13

Blethrow JD, Glavy JS, Morgan DO, Shokat KM (2008) Covalent capture of kinase-specific phosphopeptides reveals Cdk1-cyclin B substrates. Proc Natl Acad Sci U S A 105, 1442-7
18234856   Curated Info

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