Ser434
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Home > Phosphorylation Site Page: > Ser434  -  p70S6K (rat)

Site Information
SFEPKIRsPRRFIGs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448356

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 6 ) , electrophoretic mobility shift ( 4 ) , immunoprecipitation ( 1 ) , mutation of modification site ( 1 , 2 , 5 ) , peptide sequencing ( 6 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 ) , phosphoamino acid analysis ( 6 ) , phosphopeptide mapping ( 6 ) , western blotting ( 1 , 2 , 3 , 4 , 5 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 3 ) , 'neuron, hippocampal'-'brain, embryonic' ( 1 ) , 293 (epithelial) ( 5 ) , CHO (fibroblast) [EphB1 (human), transfection] ( 5 ) , COS (fibroblast) ( 2 ) , HEK293T (epithelial) ( 2 ) , L6 (myoblast) ( 4 ) , neuron-'brain, cerebral cortex' ( 2 ) , Swiss 3T3 (fibroblast) ( 6 )

Upstream Regulation
Regulatory protein:
CDK5 (rat) ( 2 ) , CDK5R1 (human) ( 2 )
Putative in vivo kinases:
CDK5 (human) ( 1 , 2 )
Kinases, in vitro:
CDK1 (human) ( 6 ) , CDK5 (human) ( 1 , 2 ) , ERK2 (human) ( 6 )
Treatments:
BDNF ( 1 ) , dexamethasone ( 4 ) , ethanol ( 3 ) , GW_8510 ( 2 ) , IGF-1 ( 3 ) , insulin ( 2 , 3 , 5 ) , rapamycin ( 1 , 2 , 5 ) , seliciclib ( 1 , 2 ) , serum ( 5 ) , wortmannin ( 5 )

Downstream Regulation
Effects of modification on p70S6K:
enzymatic activity, induced ( 2 ) , phosphorylation ( 2 )
Effects of modification on biological processes:
cytoskeletal reorganization ( 1 )

References 

1

Lai KO, et al. (2015) Cyclin-dependent Kinase 5 (Cdk5)-dependent Phosphorylation of p70 Ribosomal S6 Kinase 1 (S6K) Is Required for Dendritic Spine Morphogenesis. J Biol Chem 290, 14637-46
25903132   Curated Info

2

Hou Z, He L, Qi RZ (2007) Regulation of s6 kinase 1 activation by phosphorylation at ser-411. J Biol Chem 282, 6922-8
17220300   Curated Info

3

Kumar V, Frost RA, Lang CH (2002) Alcohol impairs insulin and IGF-I stimulation of S6K1 but not 4E-BP1 in skeletal muscle. Am J Physiol Endocrinol Metab 283, E917-28
12376318   Curated Info

4

Shah OJ, Kimball SR, Jefferson LS (2000) Glucocorticoids abate p70(S6k) and eIF4E function in L6 skeletal myoblasts. Am J Physiol Endocrinol Metab 279, E74-82
10893325   Curated Info

5

Weng QP, et al. (1998) Regulation of the p70 S6 kinase by phosphorylation in vivo. Analysis using site-specific anti-phosphopeptide antibodies. J Biol Chem 273, 16621-9
9632736   Curated Info

6

Ferrari S, et al. (1992) Activation of p70s6k is associated with phosphorylation of four clustered sites displaying Ser/Thr-Pro motifs. Proc Natl Acad Sci U S A 89, 7282-6
1496022   Curated Info