Ser44

Site Information
sGIVADLsEQsLkDG SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 468497
Available spectra: 1 CST

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 7 , 9 , 10 , 11 , 12 , 13 )
Disease tissue studied:	breast cancer ( 2 ) , cervical cancer ( 12 ) , cervical adenocarcinoma ( 12 ) , lung cancer ( 5 , 6 , 7 ) , non-small cell lung cancer ( 5 , 6 , 7 ) , non-small cell lung adenocarcinoma ( 5 , 6 , 7 )
Relevant cell line - cell type - tissue:	293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] ( 10 ) , A549 (pulmonary) ( 5 ) , BT-474 (breast cell) ( 2 ) , Cal-12T (pulmonary) ( 7 ) , Calu-3 (pulmonary) ( 6 ) , GM00130 (B lymphocyte) ( 11 ) , HCC44 (pulmonary) ( 7 ) , HCC827 (pulmonary) ( 6 ) , HeLa (cervical) ( 3 , 9 , 13 ) , HeLa S3 (cervical) ( 12 ) , liver ( 4 ) , lung ( 5 , 6 , 7 ) , NCI-H1355 (pulmonary) ( 5 ) , NCI-H1437 (pulmonary) ( 7 ) , NCI-H1650 (pulmonary) ( 6 ) , NCI-H1666 (pulmonary) ( 7 ) , NCI-H1792 (pulmonary) ( 5 ) , NCI-H1975 (pulmonary) ( 6 ) , NCI-H2106 (pulmonary) ( 6 ) , NCI-H23 (pulmonary) ( 5 ) , NCI-H2405 (pulmonary) ( 7 ) , NCI-H441 (pulmonary) ( 5 )

Upstream Regulation
Kinases, in vitro:	PLK1 (human) ( 1 )
Treatments:	ionizing_radiation ( 11 ) , nocodazole ( 12 )

References
1	Duan H, et al. (2016) Phosphorylation of PP1 Regulator Sds22 by PLK1 Ensures Accurate Chromosome Segregation. J Biol Chem 291, 21123-21136 27557660 Curated Info
2	Carrier M, et al. (2016) Phosphoproteome and Transcriptome of RA-Responsive and RA-Resistant Breast Cancer Cell Lines. PLoS One 11, e0157290 27362937 Curated Info
3	Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94 25159151 Curated Info
4	Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62 24275569 Curated Info
5	Rikova K, Hall B (2013) CST Curation Set: 20736, 21163, 30158, 30159, 30160; Year: 2013; Biosample/Treatment: cell line, A549, H1355, H23, H441, H1792; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P Curated Info
6	Rikova K, Hall B (2013) CST Curation Set: 20738, 21165, 30164, 30165, 30166; Year: 2013; Biosample/Treatment: cell line, H1650, HCC827, H1975, Calu-3, H2106; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P Curated Info
7	Rikova K, Hall B (2013) CST Curation Set: 20741, 21168, 30173, 30174, 30175; Year: 2013; Biosample/Treatment: cell line, H1666, CAL-12T, H2405, HCC44, H1437; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P Curated Info
8	Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25 22424773 Curated Info
9	Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5 21712546 Curated Info
10	Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53 20363803 Curated Info
11	Bennetzen MV, et al. (2010) Site-specific phosphorylation dynamics of the nuclear proteome during the DNA damage response. Mol Cell Proteomics 9, 1314-23 20164059 Curated Info
12	Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3 20068231 Curated Info
13	Pan C, Olsen JV, Daub H, Mann M (2009) Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomics. Mol Cell Proteomics 8, 2796-808 19651622 Curated Info