Thr21
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Home > Phosphorylation Site Page: > Thr21  -  p53 (mouse)

Site Information
ELPLsQEtFsGLWKL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447541

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 4 , 5 ) , modification-specific antibody ( 6 ) , mutation of modification site ( 3 , 5 , 6 ) , phospho-antibody ( 4 , 5 , 6 ) , western blotting ( 4 , 6 )
Disease tissue studied:
leukemia ( 4 ) , acute myelogenous leukemia ( 4 ) , cancer, squamous cell carcinoma ( 6 )
Relevant cell line - cell type - tissue:
A549 (pulmonary) ( 5 ) , bone marrow [p53 (mouse), genetic knockin] ( 3 ) , brain [p53 (mouse), genetic knockin] ( 3 ) , epidermal ( 6 ) , MEF (fibroblast) [p53 (mouse), genetic knockin] ( 3 ) , MEF (fibroblast) [PML (human)] ( 4 ) , NB-4 (myeloid) ( 4 ) , NCI-H1299 (pulmonary) ( 5 ) , testis [p53 (mouse), genetic knockin] ( 3 )

Upstream Regulation
Regulatory protein:
PML (human) ( 4 )
Putative in vivo kinases:
CK1A (human) ( 4 ) , VRK2 iso2 (human) ( 5 )
Kinases, in vitro:
VRK1 (human) ( 7 ) , VRK2 (human) ( 5 ) , VRK2 iso2 (human) ( 5 )
Treatments:
adriamycin ( 4 , 5 ) , MG132 ( 4 ) , taxol ( 5 )

Downstream Regulation
Effects of modification on p53:
activity, induced ( 3 ) , intracellular localization ( 5 ) , molecular association, regulation ( 3 , 6 ) , protein stabilization ( 5 )
Effects of modification on biological processes:
apoptosis, altered ( 3 ) , cell cycle regulation ( 3 ) , transcription, altered ( 6 )
Induce interaction with:
p300 (mouse) ( 6 )
Inhibit interaction with:
MDM2 (mouse) ( 3 , 6 )

References 

1

Wang X, et al. (2015) Pharmacologically blocking p53-dependent apoptosis protects intestinal stem cells and mice from radiation. Sci Rep 5, 8566
25858503   Curated Info

2

Song L, et al. (2011) p85α mediates p53 K370 acetylation by p300 and regulates its promoter-specific transactivity in the cellular UVB response. Oncogene 30, 1360-71
21057544   Curated Info

3

Liu D, et al. (2010) Puma is required for p53-induced depletion of adult stem cells. Nat Cell Biol 12, 993-8
20818388   Curated Info

4

Alsheich-Bartok O, et al. (2008) PML enhances the regulation of p53 by CK1 in response to DNA damage. Oncogene 27, 3653-61
18246126   Curated Info

5

Blanco S, Klimcakova L, Vega FM, Lazo PA (2006) The subcellular localization of vaccinia-related kinase-2 (VRK2) isoforms determines their different effect on p53 stability in tumour cell lines. FEBS J 273, 2487-504
16704422   Curated Info

6

Knights CD, Liu Y, Appella E, Kulesz-Martin M (2003) Defective p53 post-translational modification required for wild type p53 inactivation in malignant epithelial cells with mdm2 gene amplification. J Biol Chem 278, 52890-900
14555661   Curated Info

7

Lopez-Borges S, Lazo PA (2000) The human vaccinia-related kinase 1 (VRK1) phosphorylates threonine-18 within the mdm-2 binding site of the p53 tumour suppressor protein. Oncogene 19, 3656-64
10951572   Curated Info