Ser312
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Home > Phosphorylation Site Page: > Ser312  -  p53 (mouse)

Site Information
LPTCTsAsPPQkkkP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447530

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 16 ) , immunoprecipitation ( 15 ) , mass spectrometry ( 2 , 3 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ) , mutation of modification site ( 4 , 5 , 14 , 15 , 16 ) , phospho-antibody ( 5 ) , phosphopeptide mapping ( 16 ) , western blotting ( 5 , 14 , 15 )
Disease tissue studied:
kidney cancer ( 5 )
Relevant cell line - cell type - tissue:
3T3 (fibroblast) ( 15 ) , COS (fibroblast) ( 16 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 2 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 2 ) , HL-1 (myocyte) ( 2 ) , kidney [p53 (mouse), genetic knockin] ( 5 ) , liver [p53 (mouse), genetic knockin] ( 5 ) , MEDF [HRas (mouse), HRas V12, HDAC6 -/+] ( 7 , 8 ) , MEF (fibroblast) ( 3 , 9 , 13 ) , MEF (fibroblast) [HDAC6 (mouse), HDAC6 -/+] ( 10 , 11 ) , MEF (fibroblast) [HRas (mouse), HRas V12] ( 12 ) , MEF (fibroblast) [p53 (mouse), genetic knockin] ( 4 , 5 ) , MEF (fibroblast) [Raptor (mouse), knockdown] ( 3 ) , MEF (fibroblast) [RICTOR (mouse), knockdown] ( 3 ) , mpkCCD (renal) ( 6 ) , SF9 ( 14 ) , thymocyte [p53 (mouse), genetic knockin] ( 4 )

Upstream Regulation
Regulatory protein:
MDM4 (mouse) ( 5 )
Treatments:
ionizing_radiation ( 5 ) , UV ( 5 ) , virus infection ( 5 )

Downstream Regulation
Effects of modification on p53:
acetylation ( 14 )
Effects of modification on biological processes:
carcinogenesis, altered ( 5 ) , transcription, altered ( 5 )

Disease / Diagnostics Relevance
Relevant diseases:
lymphoma ( 5 )

References 

1

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

2

Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45
25162660   Curated Info

3

Robitaille AM, et al. (2013) Quantitative phosphoproteomics reveal mTORC1 activates de novo pyrimidine synthesis. Science 339, 1320-3
23429704   Curated Info

4

Lee MK, Tong WM, Wang ZQ, Sabapathy K (2011) Serine 312 phosphorylation is dispensable for wild-type p53 functions in vivo. Cell Death Differ 18, 214-21
20671749   Curated Info

5

Slee EA, et al. (2010) Phosphorylation of Ser312 contributes to tumor suppression by p53 in vivo. Proc Natl Acad Sci U S A 107, 19479-84
20962274   Curated Info

6

Rinschen MM, et al. (2010) Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cells. Proc Natl Acad Sci U S A 107, 3882-7
20139300   Curated Info

7

Li Y (2009) CST Curation Set: 6302; Year: 2009; Biosample/Treatment: cell line, MEDF Ras V12 HDAC6-/+/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

Li Y (2009) CST Curation Set: 6303; Year: 2009; Biosample/Treatment: cell line, MEDF Ras V12 HDAC6-/+/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

Li Y (2009) CST Curation Set: 6297; Year: 2009; Biosample/Treatment: cell line, MEF/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

Li Y (2009) CST Curation Set: 6300; Year: 2009; Biosample/Treatment: cell line, MEF HDAC6 -/+/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

11

Li Y (2009) CST Curation Set: 6301; Year: 2009; Biosample/Treatment: cell line, MEF HDAC6 -/+/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

12

Li Y (2009) CST Curation Set: 6298; Year: 2009; Biosample/Treatment: cell line, MEF Ras v12/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

13

Li Y (2009) CST Curation Set: 6296; Year: 2009; Biosample/Treatment: cell line, MEF/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

14

Warnock LJ, Raines SA, Mee TR, Milner J (2005) Role of phosphorylation in p53 acetylation and PAb421 epitope recognition in baculoviral and mammalian expressed proteins. FEBS J 272, 1669-75
15794754   Curated Info

15

Hao M, et al. (1996) Mutation of phosphoserine 389 affects p53 function in vivo. J Biol Chem 271, 29380-5
8910602   Curated Info

16

Wang Y, Eckhart W (1992) Phosphorylation sites in the amino-terminal region of mouse p53. Proc Natl Acad Sci U S A 89, 4231-5
1584757   Curated Info