Arg443

Site Information
GGLGGGMrGPPrGGM SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 48897814
Available spectra: 1 CST

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 7 )
Disease tissue studied:	lung cancer ( 6 , 7 ) , non-small cell lung cancer ( 6 , 7 ) , non-small cell lung adenocarcinoma ( 6 , 7 ) , non-small cell large cell lung carcinoma ( 7 )
Relevant cell line - cell type - tissue:	293 (epithelial) ( 2 ) , A549 (pulmonary) ( 6 ) , HCC78 (pulmonary) ( 7 ) , HEK293T (epithelial) [PADI4 (human), no information, doxycyline inducible over-expression of PADI4] ( 4 ) , HEK293T (epithelial) ( 4 ) , HeLa S3 (cervical) ( 5 ) , Jurkat (T lymphocyte) ( 3 ) , lung ( 6 , 7 ) , NCI-H1355 (pulmonary) ( 6 ) , NCI-H1781 (pulmonary) ( 7 ) , NCI-H1792 (pulmonary) ( 6 ) , NCI-H2228 (pulmonary) ( 7 ) , NCI-H23 (pulmonary) ( 6 ) , NCI-H3122 (pulmonary) ( 7 ) , NCI-H441 (pulmonary) ( 6 ) , NCI-H661 (pulmonary) ( 7 ) , U2OS (bone cell) ( 4 )

References
1	Tsai WC, et al. (2017) Histone arginine demethylase JMJD6 is linked to stress granule assembly through demethylation of the stress granule-nucleating protein G3BP1. J Biol Chem 292, 18886-18896 28972166 Curated Info
2	Larsen SC, et al. (2016) Proteome-wide analysis of arginine monomethylation reveals widespread occurrence in human cells. Sci Signal 9, rs9 27577262 Curated Info
3	Geoghegan V, et al. (2015) Comprehensive identification of arginine methylation in primary T cells reveals regulatory roles in cell signalling. Nat Commun 6, 6758 25849564 Curated Info
4	Sylvestersen KB, et al. (2014) Proteomic analysis of arginine methylation sites in human cells reveals dynamic regulation during transcriptional arrest. Mol Cell Proteomics 13, 2072-88 24563534 Curated Info
5	Bremang M, et al. (2013) Mass spectrometry-based identification and characterisation of lysine and arginine methylation in the human proteome. Mol Biosyst 9, 2231-47 23748837 Curated Info
6	Rikova K (2013) CST Curation Set: 20096; Year: 2013; Biosample/Treatment: cell line, A549, H1355, H23, H441, H1792; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: m1R Antibodies Used to Purify Peptides prior to LCMS: Mono-Methyl Arginine [mme-R] MultiMab(TM) Rabbit mAb mix Cat#: 8015 Curated Info
7	Rikova K (2013) CST Curation Set: 20100; Year: 2013; Biosample/Treatment: cell line, H2228, H3122, HCC78, H661, H1781; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: m1R Antibodies Used to Purify Peptides prior to LCMS: Mono-Methyl Arginine [mme-R] MultiMab(TM) Rabbit mAb mix Cat#: 8015 Curated Info