Ser7
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Home > Phosphorylation Site Page: > Ser7  -  CENPA (human)

Site Information
_MGPRRRsRKPEAPR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448601

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 17 ) , immunoassay ( 1 , 3 , 4 , 12 ) , immunoprecipitation ( 9 ) , microscopy-colocalization with upstream kinase ( 16 , 17 ) , mutation of modification site ( 16 , 17 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 14 , 16 , 17 , 18 ) , western blotting ( 2 , 7 , 8 , 9 , 10 , 11 , 12 , 14 , 16 )
Disease tissue studied:
cervical cancer ( 10 ) , cervical squamous cell carcinoma ( 10 ) , colorectal cancer ( 14 ) , colorectal carcinoma ( 14 ) , lymphoma ( 14 ) , B cell lymphoma ( 14 )
Relevant cell line - cell type - tissue:
BE colorectal carcinoma ( 14 ) , DT40 (B lymphocyte) ( 14 ) , HeLa (cervical) ( 2 , 3 , 4 , 7 , 8 , 9 , 11 , 12 , 16 , 17 , 18 ) , hTERT-RPE1 (epithelial) ( 1 , 6 ) , KB (squamous) ( 10 ) , U2OS (bone cell) [GR (human)] ( 18 )

Upstream Regulation
Regulatory protein:
AurB (human) ( 2 ) , Borealin (human) ( 11 ) , CENPU (human) ( 3 ) , Chk1 (human) ( 14 ) , CYLD (human) ( 9 ) , HBXAP (human) ( 2 ) , PLK1 (human) ( 2 ) , PPP2CA (human) ( 9 ) , RKIP (human) ( 15 ) , Survivin (human) ( 7 , 8 ) , TTK (human) ( 13 )
Kinases, in vitro:
AurA (human) ( 16 ) , AurB (human) ( 17 )
Treatments:
etoposide ( 10 ) , hesperadin ( 4 ) , HXDV ( 10 ) , nocodazole ( 6 , 12 , 14 ) , siRNA ( 15 ) , taxol ( 10 , 14 ) , vinblastine ( 10 ) , ZM447439 ( 2 , 4 )

Downstream Regulation
Effects of modification on CENPA:
intracellular localization ( 17 ) , molecular association, regulation ( 16 )
Effects of modification on biological processes:
cell cycle regulation ( 16 , 17 ) , chromatin organization, altered ( 16 , 17 )
Induce interaction with:
AurA (human) ( 16 )

References 

1

Papini D, Levasseur MD, Higgins JMG (2021) The Aurora B gradient sustains kinetochore stability in anaphase. Cell Rep 37, 109818
34758321   Curated Info

2

Lee HS, et al. (2021) Spatiotemporal coordination of the RSF1-PLK1-Aurora B cascade establishes mitotic signaling platforms. Nat Commun 12, 5931
34635673   Curated Info

3

Chen Q, et al. (2021) Bub1 and CENP-U redundantly recruit Plk1 to stabilize kinetochore-microtubule attachments and ensure accurate chromosome segregation. Cell Rep 36, 109740
34551298   Curated Info

4

Yi Q, et al. (2018) Aurora B kinase activity-dependent and -independent functions of the chromosomal passenger complex in regulating sister chromatid cohesion. J Biol Chem
30523151   Curated Info

5

Duan H, et al. (2016) Phosphorylation of PP1 Regulator Sds22 by PLK1 Ensures Accurate Chromosome Segregation. J Biol Chem 291, 21123-21136
27557660   Curated Info

6

Meppelink A, et al. (2015) Shugoshin-1 Balances Aurora B Kinase Activity via PP2A to Promote Chromosome Bi-orientation. Cell Rep 11, 508-15
25892238   Curated Info

7

Chu Y, et al. (2011) Aurora B kinase activation requires survivin priming phosphorylation by PLK1. J Mol Cell Biol 3, 260-7
21148584   Curated Info

8

Wang F, et al. (2010) Histone H3 Thr-3 phosphorylation by Haspin positions Aurora B at centromeres in mitosis. Science 330, 231-5
20705812   Curated Info

9

Sun L, et al. (2010) Tumour suppressor CYLD is a negative regulator of the mitotic kinase Aurora-B. J Pathol 221, 425-32
20593489   Curated Info

10

Tsai YC, et al. (2009) A G-quadruplex stabilizer induces M-phase cell cycle arrest. J Biol Chem 284, 22535-43
19531483   Curated Info

11

Bourhis E, et al. (2009) Phosphorylation of a borealin dimerization domain is required for proper chromosome segregation. Biochemistry 48, 6783-93
19530738   Curated Info

12

Klein UR, Haindl M, Nigg EA, Muller S (2009) RanBP2 and SENP3 function in a mitotic SUMO2/3 conjugation-deconjugation cycle on Borealin. Mol Biol Cell 20, 410-8
18946085   Curated Info

13

Jelluma N, et al. (2008) Mps1 phosphorylates Borealin to control Aurora B activity and chromosome alignment. Cell 132, 233-46
18243099   Curated Info

14

Zachos G, et al. (2007) Chk1 is required for spindle checkpoint function. Dev Cell 12, 247-60
17276342   Curated Info

15

Eves EM, et al. (2006) Raf kinase inhibitory protein regulates aurora B kinase and the spindle checkpoint. Mol Cell 23, 561-74
16916643   Curated Info

16

Kunitoku N, et al. (2003) CENP-A phosphorylation by Aurora-A in prophase is required for enrichment of Aurora-B at inner centromeres and for kinetochore function. Dev Cell 5, 853-64
14667408   Curated Info

17

Zeitlin SG, Shelby RD, Sullivan KF (2001) CENP-A is phosphorylated by Aurora B kinase and plays an unexpected role in completion of cytokinesis. J Cell Biol 155, 1147-57
11756469   Curated Info

18

Zeitlin SG, et al. (2001) Differential regulation of CENP-A and histone H3 phosphorylation in G2/M. J Cell Sci 114, 653-61
11171370   Curated Info

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