Thr645
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Home > Phosphorylation Site Page: > Thr645  -  MSK1 (mouse)

Site Information
PDNQPLKtPCFTLHY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448217

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 2 ) , immunoprecipitation ( 1 ) , mutation of modification site ( 4 ) , peptide sequencing ( 4 ) , phospho-antibody ( 1 , 2 , 3 , 5 ) , western blotting ( 1 , 2 , 3 , 4 , 5 )
Relevant cell line - cell type - tissue:
'brain, striatum' ( 1 ) , 293 (epithelial) ( 3 , 4 ) , heart ( 3 ) , HeLa (cervical) ( 2 ) , MEF (fibroblast) ( 2 ) , myocyte-heart ( 3 , 5 )

Upstream Regulation
Regulatory protein:
ERK2 (rat) ( 3 ) , MAPKAPK2 (human) ( 2 )
Kinases, in vitro:
ERK2 (human) ( 4 ) , P38A (human) ( 4 )
Treatments:
anisomycin ( 2 ) , endothelin ( 5 ) , GF109203X ( 5 ) , H-89 ( 5 ) , PD184352 ( 2 , 4 ) , PD98059 ( 5 ) , phenylephrine ( 5 ) , phorbol_ester ( 2 , 4 ) , prazosin ( 5 ) , propranolol ( 5 ) , RAMH ( 1 ) , SB203580 ( 2 , 4 , 5 ) , UV ( 4 )

Downstream Regulation
Effects of modification on MSK1:
enzymatic activity, induced ( 4 )

References 

1

Rapanelli M, et al. (2016) The Histamine H3 Receptor Differentially Modulates Mitogen-activated Protein Kinase (MAPK) and Akt Signaling in Striatonigral and Striatopallidal Neurons. J Biol Chem 291, 21042-21052
27510032   Curated Info

2

Ronkina N, et al. (2011) Stress induced gene expression: a direct role for MAPKAP kinases in transcriptional activation of immediate early genes. Nucleic Acids Res 39, 2503-18
21109534   Curated Info

3

Lorenz K, Schmitt JP, Schmitteckert EM, Lohse MJ (2009) A new type of ERK1/2 autophosphorylation causes cardiac hypertrophy. Nat Med 15, 75-83
19060905   Curated Info

4

McCoy CE, et al. (2007) Identification of novel phosphorylation sites in MSK1 by precursor ion scanning MS. Biochem J 402, 491-501
17117922   Curated Info

5

Markou T, Lazou A (2002) Phosphorylation and activation of mitogen- and stress-activated protein kinase-1 in adult rat cardiac myocytes by G-protein-coupled receptor agonists requires both extracellular-signal-regulated kinase and p38 mitogen-activated protein kinase. Biochem J 365, 757-63
11994045   Curated Info