Ser375
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Home > Phosphorylation Site Page: > Ser375  -  MSK1 (mouse)

Site Information
ERLFQGysFVAPsIL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448216

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 12 ) , microscopy-colocalization with upstream kinase ( 13 ) , mutation of modification site ( 10 ) , peptide sequencing ( 12 ) , phospho-antibody ( 9 , 10 , 11 , 13 , 14 , 15 , 16 , 17 , 18 ) , western blotting ( 9 , 10 , 11 , 12 , 14 , 15 , 17 , 18 )
Disease tissue studied:
breast cancer ( 14 ) , lymphoma ( 17 ) , B cell lymphoma ( 17 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 2 ) , 'neuron, hippocampal, CA1 pyramidal'-brain ( 13 ) , 293 (epithelial) ( 12 ) , 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 7 ) , 32Dcl3 (myeloid) ( 7 ) , 3T3 (fibroblast) ( 11 ) , B lymphocyte-spleen ( 17 ) , Bal 17 (B lymphocyte) ( 17 ) , brain ( 4 ) , dendritic cell ( 11 ) , dendritic cell [MAPKAPK2 (mouse), homozygous knockout] ( 11 ) , dendritic cell [MSK1 (mouse), homozygous knockout] ( 11 ) , embryo ( 4 ) , ES (stem) ( 15 ) , heart ( 3 ) , JB6 CI41 (epidermal) ( 10 ) , liver ( 4 , 5 , 8 ) , macrophage-bone marrow ( 6 , 9 ) , macrophage-bone marrow [DUSP1 (mouse), homozygous knockout] ( 6 ) , MEF (fibroblast) [IGF1R (mouse)] ( 16 ) , myocyte-heart ( 18 ) , T47D (breast cell) ( 14 )

Upstream Regulation
Regulatory protein:
ADRB1 (mouse) ( 3 ) , Cot (mouse) ( 16 )
Treatments:
anti-IgM ( 17 ) , bacterial infection ( 9 ) , BIRB-0796 ( 11 ) , contextual fear conditioning ( 13 ) , EGF ( 10 ) , endothelin ( 18 ) , GF109203X ( 18 ) , H-89 ( 18 ) , insulin ( 2 ) , LIF ( 15 ) , LPS ( 6 , 9 , 11 ) , PD184352 ( 11 ) , PD98059 ( 18 ) , phenylephrine ( 18 ) , phorbol_ester ( 10 , 12 ) , prazosin ( 18 ) , propranolol ( 18 ) , SB203580 ( 18 ) , SL327 ( 13 ) , TNF ( 16 ) , UV ( 12 )

Downstream Regulation
Effects of modification on MSK1:
enzymatic activity, induced ( 13 )

References 

1

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

2

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

3

Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11
23737553   Curated Info

4

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

5

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

6

Weintz G, et al. (2010) The phosphoproteome of toll-like receptor-activated macrophages. Mol Syst Biol 6, 371
20531401   Curated Info

7

Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39
19854140   Curated Info

8

Zhou J (2009) CST Curation Set: 7425; Year: 2009; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: HXXp[ST]
Curated Info

9

Leng J, et al. (2009) Toxoplasma gondii prevents chromatin remodeling initiated by TLR-triggered macrophage activation. J Immunol 182, 489-97
19109180   Curated Info

10

Kim HG, et al. (2008) Mitogen- and stress-activated kinase 1-mediated histone H3 phosphorylation is crucial for cell transformation. Cancer Res 68, 2538-47
18381464   Curated Info

11

Zaru R, et al. (2007) The MAPK-activated kinase Rsk controls an acute Toll-like receptor signaling response in dendritic cells and is activated through two distinct pathways. Nat Immunol 8, 1227-35
17906627   Curated Info

12

McCoy CE, et al. (2007) Identification of novel phosphorylation sites in MSK1 by precursor ion scanning MS. Biochem J 402, 491-501
17117922   Curated Info

13

Sindreu CB, Scheiner ZS, Storm DR (2007) Ca2+ -stimulated adenylyl cyclases regulate ERK-dependent activation of MSK1 during fear conditioning. Neuron 53, 79-89
17196532   Curated Info

14

Vicent GP, et al. (2006) Induction of progesterone target genes requires activation of Erk and Msk kinases and phosphorylation of histone H3. Mol Cell 24, 367-81
17081988   Curated Info

15

Lee ER, McCool KW, Murdoch FE, Fritsch MK (2006) Dynamic changes in histone H3 phosphoacetylation during early embryonic stem cell differentiation are directly mediated by mitogen- and stress-activated protein kinase 1 via activation of MAPK pathways. J Biol Chem 281, 21162-72
16728397   Curated Info

16

Das S, et al. (2005) Tpl2/cot signals activate ERK, JNK, and NF-kappaB in a cell-type and stimulus-specific manner. J Biol Chem 280, 23748-57
15833743   Curated Info

17

Blois JT, et al. (2004) B cell receptor-induced cAMP-response element-binding protein activation in B lymphocytes requires novel protein kinase Cdelta. J Biol Chem 279, 30123-32
15138267   Curated Info

18

Markou T, Lazou A (2002) Phosphorylation and activation of mitogen- and stress-activated protein kinase-1 in adult rat cardiac myocytes by G-protein-coupled receptor agonists requires both extracellular-signal-regulated kinase and p38 mitogen-activated protein kinase. Biochem J 365, 757-63
11994045   Curated Info