Ser334

Site Information
LQGsRkGsLssVtPs SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 4776523

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 , 6 , 8 , 9 )
Disease tissue studied:	breast cancer ( 6 ) , lung cancer ( 6 , 8 ) , non-small cell lung cancer ( 6 ) , melanoma skin cancer ( 2 )
Relevant cell line - cell type - tissue:	BT-549 (breast cell) ( 6 ) , CL1-0 (pulmonary) ( 8 ) , CL1-1 (pulmonary) ( 8 ) , CL1-2 (pulmonary) ( 8 ) , CL1-5 (pulmonary) ( 8 ) , HCC2279 (pulmonary) ( 6 ) , HeLa (cervical) ( 1 , 3 , 9 ) , liver ( 4 ) , MDA-MB-231 (breast cell) ( 6 ) , NCI-H2172 (pulmonary) ( 6 ) , WM239A (melanocyte) ( 2 )

Upstream Regulation
Treatments:	EGF ( 9 ) , metastatic potential ( 8 )

References
1	Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296 27281782 Curated Info
2	Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615 25850435 Curated Info
3	Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94 25159151 Curated Info
4	Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62 24275569 Curated Info
5	Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21 23312004 Curated Info
6	Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68 22617229 Curated Info
7	Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25 22424773 Curated Info
8	Wang YT, et al. (2010) An informatics-assisted label-free quantitation strategy that depicts phosphoproteomic profiles in lung cancer cell invasion. J Proteome Res 9, 5582-97 20815410 Curated Info
9	Pan C, Olsen JV, Daub H, Mann M (2009) Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomics. Mol Cell Proteomics 8, 2796-808 19651622 Curated Info