Tyr1472
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Home > Phosphorylation Site Page: > Tyr1472  -  NMDAR2B (mouse)

Site Information
GSSNGHVyEKLSSIE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448167
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 ) , mass spectrometry ( 9 , 11 , 12 ) , mutation of modification site ( 8 , 13 , 15 ) , phospho-antibody ( 2 , 3 , 4 , 6 , 7 , 8 , 10 , 13 , 14 , 15 ) , phosphopeptide mapping ( 16 ) , western blotting ( 2 , 3 , 4 , 6 , 7 , 8 , 10 )
Relevant cell line - cell type - tissue:
'brain, cerebral cortex' ( 7 ) , 'brain, embryonic' ( 11 ) , 'brain, hippocampus' ( 13 ) , 'brain, hippocampus' [Fyn (mouse)] ( 16 ) , 'brain, striatum' ( 8 ) , 293 (epithelial) ( 2 , 10 ) , brain ( 4 , 9 , 12 ) , HEK293T (epithelial) [Fyn (mouse)] ( 16 ) , HEK293T (epithelial) ( 15 ) , neuron ( 10 ) , neuron-'brain, hippocampus' ( 3 ) , neuron:synaptosome-'brain, cerebral cortex' ( 2 ) , spinal cord ( 6 , 14 )

Upstream Regulation
Regulatory protein:
CDK5 (human) ( 10 ) , PSD-95 (human) ( 10 )
Putative in vivo kinases:
Fyn (mouse) ( 14 , 16 ) , Src (mouse) ( 10 )
Putative upstream phosphatases:
STEP (mouse) ( 7 )
Treatments:
activin ( 3 ) , beta-amyloid ( 7 ) , colforsin ( 6 ) , electrical_stimulation ( 13 ) , EP1-selective_antagonist ( 14 ) , ephrin_B2 ( 15 ) , forced swim test ( 8 ) , Freund's_complete_adjuvant ( 6 ) , H-89 ( 6 ) , indometacin ( 14 ) , ketamine ( 4 ) , PP2 ( 6 ) , seliciclib ( 10 ) , SU6656 ( 10 )

Downstream Regulation
Effects of modification on NMDAR2B:
activity, induced ( 13 ) , intracellular localization ( 7 , 14 ) , phosphorylation ( 13 ) , receptor internalization, altered ( 3 )

Disease / Diagnostics Relevance
Relevant diseases:
Alzheimer's disease ( 7 )

References 

1

McKay S, et al. (2018) The Developmental Shift of NMDA Receptor Composition Proceeds Independently of GluN2 Subunit-Specific GluN2 C-Terminal Sequences. Cell Rep 25, 841-851.e4
30355491   Curated Info

2

Lu W, et al. (2015) Phosphorylation of Tyrosine 1070 at the GluN2B Subunit Is Regulated by Synaptic Activity and Critical for Surface Expression of N-Methyl-d-aspartate (NMDA) Receptors. J Biol Chem 290, 22945-54
26229100   Curated Info

3

Lau D, Bengtson CP, Buchthal B, Bading H (2015) BDNF Reduces Toxic Extrasynaptic NMDA Receptor Signaling via Synaptic NMDA Receptors and Nuclear-Calcium-Induced Transcription of inhba/Activin A. Cell Rep 12, 1353-66
26279570   Curated Info

4

Long LE, et al. (2015) Neuregulin 1 expression and electrophysiological abnormalities in the Neuregulin 1 transmembrane domain heterozygous mutant mouse. PLoS One 10, e0124114
25992564   Curated Info

5

Nakai T, et al. (2014) Girdin Phosphorylation Is Crucial for Synaptic Plasticity and Memory: A Potential Role in the Interaction of BDNF/TrkB/Akt Signaling with NMDA Receptor. J Neurosci 34, 14995-5008
25378165   Curated Info

6

Yang HB, et al. (2011) cAMP-dependent protein kinase activated Fyn in spinal dorsal horn to regulate NMDA receptor function during inflammatory pain. J Neurochem 116, 93-104
21054385   Curated Info

7

Kurup P, et al. (2010) Abeta-mediated NMDA receptor endocytosis in Alzheimer's disease involves ubiquitination of the tyrosine phosphatase STEP61. J Neurosci 30, 5948-57
20427654   Curated Info

8

Taniguchi S, et al. (2009) Involvement of NMDAR2A tyrosine phosphorylation in depression-related behaviour. EMBO J 28, 3717-29
19834457   Curated Info

9

Zhou J (2008) CST Curation Set: 4322; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY
Curated Info

10

Zhang S, et al. (2008) Cdk5 regulates the phosphorylation of tyrosine 1472 NR2B and the surface expression of NMDA receptors. J Neurosci 28, 415-24
18184784   Curated Info

11

Guo A (2007) CST Curation Set: 2672; Year: 2007; Biosample/Treatment: tissue, brain/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

12

Guo A (2007) CST Curation Set: 2675; Year: 2007; Biosample/Treatment: tissue, brain/LY; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

13

Nakazawa T, et al. (2006) NR2B tyrosine phosphorylation modulates fear learning as well as amygdaloid synaptic plasticity. EMBO J 25, 2867-77
16710293   Curated Info

14

Abe T, et al. (2005) Fyn kinase-mediated phosphorylation of NMDA receptor NR2B subunit at Tyr1472 is essential for maintenance of neuropathic pain. Eur J Neurosci 22, 1445-54
16190898   Curated Info

15

Takasu MA, Dalva MB, Zigmond RE, Greenberg ME (2002) Modulation of NMDA receptor-dependent calcium influx and gene expression through EphB receptors. Science 295, 491-5
11799243   Curated Info

16

Nakazawa T, et al. (2001) Characterization of Fyn-mediated tyrosine phosphorylation sites on GluR epsilon 2 (NR2B) subunit of the N-methyl-D-aspartate receptor. J Biol Chem 276, 693-9
11024032   Curated Info