Thr386

Site Information
IGLNQPstPtHAASI SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 448288

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 , 5 ) , mutation of modification site ( 6 ) , western blotting ( 6 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 1 ) , 'brain, embryonic' ( 4 ) , 'neuron, hippocampal, CA1 pyramidal'-brain ( 2 ) , brain ( 3 ) , heart ( 5 ) , PS120 (fibroblast) ( 6 )

Upstream Regulation
Putative in vivo kinases:	ERK2 (mouse) ( 6 )
Kinases, in vitro:	ERK1 (mouse) ( 6 ) , ERK2 (mouse) ( 6 )

References
1	Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6 26060331 Curated Info
2	Li L, et al. (2009) Serotonin(1A)-receptor-dependent signaling proteins in mouse hippocampus. Neuropharmacology 57, 556-66 19607848 Curated Info
3	Zhou J (2008) CST Curation Set: 3807; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P , PTMScan(R) PLK Binding Motif (SpTP) Immunoaffinity Beads Cat#: 1995 Curated Info
4	Zhou J (2008) CST Curation Set: 3808; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P , PTMScan(R) PLK Binding Motif (SpTP) Immunoaffinity Beads Cat#: 1995 Curated Info
5	Zhou J (2008) CST Curation Set: 3809; Year: 2008; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P , PTMScan(R) PLK Binding Motif (SpTP) Immunoaffinity Beads Cat#: 1995 Curated Info
6	Brunet A, Pagès G, Pouysségur J (1994) Growth factor-stimulated MAP kinase induces rapid retrophosphorylation and inhibition of MAP kinase kinase (MEK1). FEBS Lett 346, 299-303 8013650 Curated Info