Ser221
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Home > Phosphorylation Site Page: > Ser221  -  GR (mouse)

Site Information
DLEFSAGsPGKETNE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447967

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 5 ) , immunoprecipitation ( 3 ) , mass spectrometry ( 1 ) , mutation of modification site ( 3 ) , peptide sequencing ( 5 ) , phospho-antibody ( 2 , 3 ) , phosphoamino acid analysis ( 4 ) , phosphopeptide mapping ( 5 ) , western blotting ( 2 , 3 )
Relevant cell line - cell type - tissue:
CHO (fibroblast) [EphB1 (human), transfection] ( 5 ) , LbetaT2 ( 2 ) , liver ( 1 ) , MEF (fibroblast) ( 3 ) , WCL2 (epithelial) ( 4 ) , WEHI-7 (T lymphocyte) ( 5 )

Upstream Regulation
Regulatory protein:
FLOT1 (mouse) ( 2 )
Putative upstream phosphatases:
PPP5C (human) ( 3 )
Treatments:
dexamethasone ( 2 ) , GnRH ( 2 ) , triamcinolone_acetonide ( 4 )

Downstream Regulation
Effects of modification on biological processes:
transcription, induced ( 3 )

References 

1

Robles MS, Humphrey SJ, Mann M (2017) Phosphorylation Is a Central Mechanism for Circadian Control of Metabolism and Physiology. Cell Metab 25, 118-127
27818261   Curated Info

2

Wehmeyer L, Du Toit A, Lang DM, Hapgood JP (2014) Lipid Raft- and Protein Kinase C-mediated Synergism between Glucocorticoid- and Gonadotropin-releasing Hormone Signaling Results in Decreased Cell Proliferation. J Biol Chem 289, 10235-51
24558046   Curated Info

3

Hinds TD, et al. (2011) Protein phosphatase 5 mediates lipid metabolism through reciprocal control of glucocorticoid receptor and peroxisome proliferator-activated receptor-γ (PPARγ). J Biol Chem 286, 42911-22
21994940   Curated Info

4

Bodwell JE, Hu JM, Orti E, Munck A (1995) Hormone-induced hyperphosphorylation of specific phosphorylated sites in the mouse glucocorticoid receptor. J Steroid Biochem Mol Biol 52, 135-40
7873448   Curated Info

5

Bodwell JE, et al. (1991) Identification of phosphorylated sites in the mouse glucocorticoid receptor. J Biol Chem 266, 7549-55
2019585   Curated Info

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